Investigating the Production of Lipoproteins and Acetyl-CoA During a Ketogenic Diet

Investigating the Production of Lipoproteins and Acetyl-CoA During a Ketogenic Diet

The purpose of this study is to understand how the reduction in dietary carbohydrates in a very-low carbohydrate ketogenic diet impacts the synthesis of cholesterol, fatty acid, and ketones, and the turnover rate of VLDL and chylomicron particles.

Participants will consume a standard American diet for one week followed by a very-low carbohydrate ketogenic diet (VLCKD, <50 grams carbohydrate/day) for two weeks. Participants will undergo an oral stable isotope tracer study during each diet to measure de novo synthesis of fatty acid, cholesterol, ketone bodies, and for measurement of apolipoprotein B and triglyceride kinetics.

Inclusion Criteria:

• Male
• 18-50 years old

Exclusion Criteria:

• Use of nicotine products
• Has dietary restrictions
• Lactose intolerance
• History of GI disorders, diabetes, liver, kidney, or thyroid disorders
• Taking hypolipidemic, anti-diabetic, anti-hypertensive, or anti-depression medication
• Screening plasma triglyceride < 50mg/dl or >500mg/dl
• Screening glucose > 125mg/dl
• Screening total and LDL-Cholesterol over 95th %tile for age and sex
• BP > 160/95 mmHg
• Weight loss/gain > 5% in the previous 3 months (self-reported)