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| ID | Type | Description | Link |
|---|---|---|---|
| UAB - Urology | Other Identifier | UAB - Urology |
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The purpose of this study is to evaluate mitochondrial function in white blood cells and platelets from healthy individuals following dietary oxalate intake.
Inflammation and dietary oxalate have been shown to play an important role in the development of kidney stones. Oxalate is a small molecule found in plants and plant-derived food. Individuals that have high oxalate intake have an increased risk of developing kidney stones. The co-investigators have previously shown that healthy subjects that consume dietary oxalate have increased plasma and urine oxalate.
White blood cells are essential for the immune response and rely on the mitochondria to carry out important cell functions. The Principal Investigator's current research shows that patients with calcium oxalate kidney stones have decreased mitochondrial function in their immune cells. Using the expertise of the investigators, this study will test whether oxalate has a direct effect on mitochondrial function in immune cells from healthy subjects. This information could help us understand the role of oxalate on the immune system during kidney stone pathogenesis.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 3d Diet+Spinach Smoothie+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to study site and drink a prepared blended spinach smoothie and be provided a breakfast meal. |
|
| 5d Diet+Spinach Smoothie+Breakfast | Experimental | 5 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie and be provided a breakfast meal. |
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| 3d Diet+Kale Smoothie+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On the final day, participants will return to the study site and drink a prepared kale smoothie and be provided a breakfast meal. |
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| 3d Diet+V Spinach Smoothie+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie with varying amounts of spinach and be provided a breakfast meal. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Breakfast | Dietary Supplement | a prepared meal consisting of pastry and 2 cooked egg whites |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change in monocyte cellular energetics after 5 hours | Cellular bioenergetics (oxygen consumption rate) will be measured in isolated monocytes from human blood using a Seahorse XF Analyzer machine. Monocyte cellular bioenergetics will be measured before and after consuming a spinach smoothie (pre- and post-loads; Time 0 hours and 5 hours) to assess a change in monocyte cellular energetics after 5 hours. | 0 hours - 5 hours |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Tanecia Mitchell, PhD | University of Alabama at Birmingham | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Alabama at Birmingham | Birmingham | Alabama | 35233 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33732242 | Derived | Kumar P, Patel M, Oster RA, Yarlagadda V, Ambrosetti A, Assimos DG, Mitchell T. Dietary Oxalate Loading Impacts Monocyte Metabolism and Inflammatory Signaling in Humans. Front Immunol. 2021 Feb 25;12:617508. doi: 10.3389/fimmu.2021.617508. eCollection 2021. |
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| ID | Term |
|---|---|
| D007669 | Kidney Calculi |
| ID | Term |
|---|---|
| D053040 | Nephrolithiasis |
| D007674 | Kidney Diseases |
| D014570 | Urologic Diseases |
| D052776 | Female Urogenital Diseases |
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| ID | Term |
|---|---|
| D062408 | Breakfast |
| ID | Term |
|---|---|
| D062407 | Meals |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
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| 3d Diet+Blended Smoothie+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended smoothie and be provided a breakfast meal. |
|
| 3d Diet+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and be provided a breakfast meal. |
|
| 3d Diet+Sodium Oxalate Drink+Breakfast | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared sodium oxalate drink and be provided a breakfast meal. |
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| 3d Diet+Spinach Smoothie+Breakfast w/ 24 Hr Urine | Experimental | 3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a blended spinach smoothie and be provided a breakfast meal. |
|
| Spinach smoothie | Dietary Supplement | a prepared blended spinach smoothie, consisting of banana, avocado, orange juice, and spinach |
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| Kale smoothie | Dietary Supplement | prepared blended kale smoothie, consisting of banana, avocado, orange juice, and kale |
|
| Blended smoothie | Dietary Supplement | a prepared blended smoothie, consisting of banana, avocado, and orange juice |
|
| V spinach smoothie | Dietary Supplement | a prepared blended spinach smoothie, consisting of banana, avocado, orange juice, and a varying amount (low, medium, high) of spinach |
|
| Sodium oxalate drink | Dietary Supplement | a prepared sodium oxalate drink |
|
| D005261 |
| Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D052878 | Urolithiasis |
| D014545 | Urinary Calculi |
| D052801 | Male Urogenital Diseases |
| D002137 | Calculi |
| D020763 | Pathological Conditions, Anatomical |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D019602 |
| Food and Beverages |