Contribution of PRF in CDH in Children With Prothetic Patch Closure

Contribution of PRF (Platelet Rich Fibrin) in the Biological Functionalization of Prothetic Patch Closure : in Vitro Study

Improved management of giant congenital diaphragmatic hernias (CDH) in neonates : decreased risk of morbidity and mortality due to prosthesis release. CDH is a rare disease with a still very dark prognosis, with a high rate of morbidity and mortality in giants forms linked to the release of insufficiently biologically integrated prosthesis. The biological functionalization of the prosthetic materials by host PRF would improve the biological colonization of materials and thus reduce the risk of prosthetic release.

Inclusion Criteria:

Adult Healthy Volunteers :

- Over 18 years of age

Neonates:

• Aged between 1h of life and 28 days of life
• Born beyond 33 Week of Amenorrhea + 1day and 2kg of birth weight
• Hospitalized in the medical-surgical centre of Pediatrics of the hospital of Strasbourg
• For whom a blood sample was prescribed as part of their routine care

Exclusion Criteria:

Adult Healthy Volunteers :

• Systemic inflammatory disease
• Transient inflammatory state
• Any drug that modifies the coagulation cascade during the 48h preceding the sampling

Neonates:

• Risk of anemia < 7g/DL
• Current anticoagulant treatment