Analysis of in Vitro Biological Reaction of Diaphragmatic Prothesis When Congenital Diaphragmatic Hernia (CDH) Repair

Mecanical Properties of Biomaterials and Biological Integration of Diaphragmatic Prothesis in Infancy

In case of CDH, the incomplete development of a part of the diaphragm allows abdominal viscera to herniate into thorax cavity during fetal development and impaired lung and heart development.

After birth, CDH patients who survived thanks to an active reanimation management undergo diaphragmatic repair. In case of large defect, a patch is needed to complete diaphragmatic closure. Thanks to primary cell culture from foreskin, peritoneal epithelium or cremaster dedicated to incineration investigators want to analyse biological integration of actually used prothesis and others innovations.

Inclusion Criteria:

• Minor aged 0 (day of birth) to 14 years
• Patient for whom an indication of posthectomy and / or cure of inguinal hernia and / or testicular ectopy is retained
• Patient whose legal representatives have agreed to the use of operational waste from mesenchymal tissues (foreskin, cremaster and peritoneum) destined for incineration.
• Patient affiliated to a social security scheme

Exclusion Criteria:

- parents don't agree to the use of its operating waste for research purposes