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The aim of this study is to compare prepuce tissue samples from healthy male children who applied for circumcision and children with isolated hypospadias. Estrogen receptor 1 (ER1), androgen receptor (AR), FGFR2, transforming growth factor beta (TGF beta) and HOXA13 gene expression in the prepuce is planned to investigate via polymerase chain reaction (PCR) method. Mucosal vascular and nerve densities, and extracellular matrix density of laminin and collagen protein is planned to investigate via immunohistochemical examination.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Study | Children with isolated hypospadias | ||
| Control | Children with planned circumcision |
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| Measure | Description | Time Frame |
|---|---|---|
| Genetic analysis 1 | Androgen receptor (AR) gene PCR analysis | 1 year |
| Genetic analysis 2 | Estrogen receptor 1 (ER1) gene PCR analysis | 1 year |
| Genetic analysis 3 | FGFR2 gene PCR analysis | 1 year |
| Genetic analysis 4 | TGF beta gene PCR analysis | 1 year |
| Genetic analysis 5 | HOXA13 gene PCR analysis | 1 year |
| Immunohistochemical study 1 | Mucosal vessel density is planned to determine via CD34 | 1 year |
| Immunohistochemical study 2 | Mucosal nerve density is planned to determine via PGP 9.5 | 1 year |
| Histological study 1 | Laminin is planned to determine in extracellular matrix via light microscope | 1 year |
| Histological study 2 | Collagen is planned to determine in extracellular matrix via light microscope |
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Inclusion Criteria:
Exclusion Criteria:
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0-18 years old pediatric male population, patients with isolated hypospadias are study group, patients with planned circumcision are control group.
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| Name | Affiliation | Role |
|---|---|---|
| Dincer Avlan, Profesor, MD | Trakya University Faculty of Medicine, Pediatric Surgery Department | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Trakya University Faculty of Medicine | Edirne | 22030 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D007021 | Hypospadias |
| D003966 | Camurati-Engelmann Syndrome |
| ID | Term |
|---|---|
| D014564 | Urogenital Abnormalities |
| D052776 | Female Urogenital Diseases |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
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Preputial tissue
| 1 year |
| D010409 | Penile Diseases |
| D005832 | Genital Diseases, Male |
| D000091662 | Genital Diseases |
| D052801 | Male Urogenital Diseases |
| D000013 | Congenital Abnormalities |
| D009358 | Congenital, Hereditary, and Neonatal Diseases and Abnormalities |
| D010009 | Osteochondrodysplasias |
| D001848 | Bone Diseases, Developmental |
| D001847 | Bone Diseases |
| D009140 | Musculoskeletal Diseases |
| D030342 | Genetic Diseases, Inborn |