Immunophenotyping of Blast Cells and Immune Effector Cells in Peripheral Whole Blood and Bone Marrow Samples From AML and MDS Patients (AML)

Immunophenotyping of Blast Cells and Immune Effector Cells in Peripheral Whole Blood and Bone Marrow Samples From AML and MDS Patients

The objective of this study is to evaluate the immune profile of blast cells and immune effector cells in paired peripheral whole blood and bone marrow samples from AML and MDS patients by standardized flow cytometry. A special emphasis will be focused on monitoring expression of CD200 as well as PGP-170 (MDR1) on blasts cells.

Inclusion Criteria:

• Age > 18
• No pregnant woman
• Patient with myelodysplastic syndrome (with excess blasts) or acute myeloid leukemia
• Presence of a monitored serum blastose
• Patient who can obtain free and informed consent (speaks French, no guardianship or curatorship)

Exclusion Criteria:

• Minor Patient (< 18 years old)
• Medical or psychological Condition that could interact with the ability to understand the study,
• Pregnant or lactating women,
• Major persons under guardianship or under the protection of justice
• Persons deprived of their liberty by a judicial or administrative decision
• Lack of information and opposition to its participation in the study