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Determine the efficacy of the investigational product versus placebo in reducing stress oxidative during the performance of a physical exercise of a certain intensity and duration.
The effectiveness of a probiotic for oxidative stress after eight weeks of the product to study will be checked. the sample will be formed by cyclists that will submit to a baseline exercise and after the product is taken.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Experimental | Experimental | One capsule a day will be consumed. At breakfast for the six weeks. |
|
| Comparator | Placebo Comparator | One capsule a day will be consumed. At breakfast for the six weeks. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Lactobacillus rhamnosus, Lactobacillus casei y Bifidobacterium longum (100MG) | Dietary Supplement | six weeks of consumption |
|
| Measure | Description | Time Frame |
|---|---|---|
| Oxidative damage to lipids | It will be measured by isoprostanes in urine 24 hours. | Oxidative lipid damage will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Measure | Description | Time Frame |
|---|---|---|
| Oxidative damage to DNA | Analysis of 8-oxo 2'-deoxyguanosine in 24-hour urine. | Oxidative damage to DNA will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Oxidative damage to proteins. |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Catholic University of Murcia | Murcia | 30107 | Spain |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33671691 | Derived | Sanchez Macarro M, Avila-Gandia V, Perez-Pinero S, Canovas F, Garcia-Munoz AM, Abellan-Ruiz MS, Victoria-Montesinos D, Luque-Rubia AJ, Climent E, Genoves S, Ramon D, Chenoll E, Lopez-Roman FJ. Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise. Antioxidants (Basel). 2021 Feb 22;10(2):323. doi: 10.3390/antiox10020323. |
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| Lactobacillus rhamnosus, Lactobacillus casei y Bifidobacterium longum (300MG) | Dietary Supplement | six weeks of consumption |
|
| maltodextrin and sucrose (PLACEBO) | Dietary Supplement | six weeks of consumption |
|
Analysis of serum carbonyl groups |
| Oxidative damage to protein will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Oxidative damage to lipids | analysis of malondialdehyde in plasma | Oxidative damage to lipids will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Blood analysis | serum carbonyls. It is measured in nmol / mg proteins | Blood analysis will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Blood analysis | Malondialdehyde in plasma | Blood analysis will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Blood analysis | Oxidized LDL. | Blood analysis will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Blood analysis | Antioxidant capacity. | Blood analysis will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| Blood analysis | glutathione metabolism. It is measured in nmol / mg proteins | Blood analysis will be measured by the ELISA test on two different occasions. The measurements will be made at the beginning and at six weeks of consumption of the product. |
| ID | Term |
|---|---|
| C008315 | maltodextrin |
| D013395 | Sucrose |
| ID | Term |
|---|---|
| D004187 | Disaccharides |
| D009844 | Oligosaccharides |
| D011134 | Polysaccharides |
| D002241 | Carbohydrates |
| D000073893 | Sugars |
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