The purpose of this study is to determine if short-term adoption of a Westernized lifestyle characterized by physical inactivity and increased consumption of fructose will result in metabolic and vascular dysfunction. Healthy individuals aged 18-45 years old will undergo an acute period of physical inactivity (10 days) coupled with increased fructose consumption. Augmented fructose consumption will be achieved via commercially available soda beverages which are high in fructose. Vascular and metabolic function measures will be performed before and after the 10-day intervention.

Men and women who regularly engage in >10,000 steps per day, age 18-45 years, body mass index < 30 kg/m2, and with no history of chronic disease will be recruited. Subjects who are eligible will undergo an acute period of physical inactivity (10 days) coupled with increased fructose consumption (6 cans/day). Physical activity levels will be assessed via a pedometer to quantify if individuals achieve <5000 steps/day. Vascular and metabolic function measures will be performed before and after the 10-day intervention.

Inclusion Criteria:

• Men and women who regularly engage in >10,000 steps per day
• Age 18-45 years
• Body mass index < 30 kg/m2
• No history of chronic disease

Exclusion Criteria:

• Known cardiovascular disease including hypertension, heart failure, coronary artery disease, and stroke
• Renal or hepatic diseases
• Active cancer
• Autoimmune diseases
• Current use of immunosuppressant therapy
• Excessive alcohol consumption (> 14 drinks/week for men and >7 drinks/week for women)
• Current tobacco use
• Current pregnancy
• Diagnosis of type 2 diabetes
• Regular consumption of >2 non-diet sodas/day