Study the Effect of Blood Transfusion on Oxidant-antioxidant Status in Term and Near-term Neonates

Study the Effect of Blood Transfusion on Oxidant-antioxidant Status in Term and Near-term Neonates

Primary aim is to evaluate the effect of blood transfusion on oxidant-antioxidant status in premature neonates. Secondary aim is to assess the effect of the age of transfused red blood cells on the biological markers of oxidative stress.

Oxidant-antioxidant status will be assessed by measuring total antioxidant capacity (TAC) and malondialdehyde (MDA), as well as antioxidant minerals, in 65 term and near-term neonates.

Inclusion Criteria:

• Premature neonates ≤36 weeks in need for blood transfusion.

Exclusion Criteria:

• Critically ill neonates.(perinatal asphaxia,on high setting mechanical ventilation,different types of shock eg ;septic shock and major congenital anomalies)
• Extremely low birth weight neonates(<1000gm birth weight )
• Recipient of blood transfusion before enrollment in the study.
• Neonates planned for exchange transfusion .