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Prevalence rates of childhood obesity have reached alarming levels. As childhood obesity may already be associated with serious comorbidities, obese adolescents are at significantly higher risk for obesity and increased morbidity and mortality during adulthood. Combined lifestyle interventions, which include regular physical activity and dietary restriction, have been shown to result in most significant improvements in cardiovascular function and their associated factors in the pediatric and adolescent population with obesity. The aim of this study is to investigate the effects of a combined exercise and diet intervention on cardiovascular function and their associated factors in obese children.
Sixty obese children and adolescents will complete a 6-week program of exercise and diet intervention. Thirty normal-weight children and adolescents will be recruited as control group. Clinical characteristics, body composition, blood biochemistry, and circulating irisin levels of the subjects will be measured before and after 6-week intervention. Endothelial function will be assessed by a flow-mediated dilation test. Circulating exosome-derived miRNAs, exercise-induced epigenetic modifications, circulating irisin levels irisin concentration in plasma and arterial stiffness as well as cardiac autonomic function will be investigated before and after the 6-week intervention. Fecal samples were collected before and after the 6-week intervention for analysis of the compositional and functional changes in the human gut microbiota.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Obese intervention group | Experimental | Obese children were received exercise and diet intervention for 6 weeks. |
|
| Normal weight control group | No Intervention | Normal weight children were recruited as a control group without any intervention. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Exercise | Behavioral | Subjects were provided with energy-restricted diets of 1300-2200 kcal/day based on weight. The energy percentages provided by protein, fat and carbohydrate were 20%, 20% and 60%, respectively, while energy distributions at breakfast, lunch and dinner were 30%, 40% and 30%, respectively. Subjects performed a series of endurance exercise such as bicycling, walking, running, dancing and ball games for 5 hour/day, supplemented with resistance exercise. Training interventions were performed 5-6 day/week for 6 weeks. The exercise program was designed to result in an energy expenditure of 1500-2500 kcal/day. |
| Measure | Description | Time Frame |
|---|---|---|
| Change from flow-mediated dilation (FMD) at 6 weeks | for brachial endothelial function assessment | before and after the 6-week intervention |
| Measure | Description | Time Frame |
|---|---|---|
| Change from circulating exosome-derived miRNAs at 6 weeks | exosome-secreted miRNA levels in plasma | before and after the 6-week intervention |
| Change from circulating irisin levels at 6 weeks | irisin concentration in plasma |
| Measure | Description | Time Frame |
|---|---|---|
| Change from the composition of gut microbiota at 6 weeks | the composition of gut microbiota in fecal samples | before and after the 6-week intervention |
| Change from short chain fatty acids (SCFAs) concentractions at 6 weeks |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Junhao Huang, PhD | Contact | +86 (0)20 3802 5087 | junhaohuang2006@hotmail.com | |
| Dan Wang | Contact | +86 (0)20 3802 5087 | 17880383@qq.com |
| Name | Affiliation | Role |
|---|---|---|
| Min Hu, PhD | Guangzhou Sport University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Guangzhou Sport University | Recruiting | Guangzhou | Guangdong | 510000 | China |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33365012 | Derived | Huang J, Liao J, Fang Y, Deng H, Yin H, Shen B, Hu M. Six-Week Exercise Training With Dietary Restriction Improves Central Hemodynamics Associated With Altered Gut Microbiota in Adolescents With Obesity. Front Endocrinol (Lausanne). 2020 Dec 7;11:569085. doi: 10.3389/fendo.2020.569085. eCollection 2020. | |
| 32072570 | Derived |
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| ID | Term |
|---|---|
| D063766 | Pediatric Obesity |
| ID | Term |
|---|---|
| D009765 | Obesity |
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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|
|
| before and after the 6-week intervention |
| Change from pulse wave velocity at 6 weeks | for arterial stiffness assessment | before and after the 6-week intervention |
| Change from heart rate variability at 6 weeks | for cardiac autonomic function assessment | before and after the 6-week intervention |
short chain fatty acids (SCFAs) concentractions in fecal samples
| before and after the 6-week intervention |
| Liao J, Huang J, Wang S, Xiang M, Wang D, Deng H, Yin H, Xu F, Hu M. Effects of exercise and diet intervention on appetite-regulating hormones associated with miRNAs in obese children. Eat Weight Disord. 2021 Mar;26(2):457-465. doi: 10.1007/s40519-020-00869-9. Epub 2020 Feb 18. |
| D009750 |
| Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |