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| Name | Class |
|---|---|
| Banoon IVF Center | OTHER |
| Quena IVF Centre | UNKNOWN |
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Any drift in extracellular pH (pHe) from the intercellular pH (pHi) could alter the mechanism regulating transporters and exchangers through the plasma membrane. This could lead to difficulty in restoring the physiological level of pHi. The critical stages that lack robust mechanism of regulating the pHi are the denuded oocyte, early stage of fertilization or a thawed embryo. This, in turn, could lead to a significant effect of the perturbations on embryo development. Convincing evidence regarding the optimal pHe for culturing human embryo is, so far, scanty and mostly anecdotal. The embryo has been reported to have a compensatory mechanism to grow at pHe between 7.0-7.4. Others have recommended that embryo need a sequential pH to viably grow suggesting that the post-genomic activation stage needs a slightly alkaline range of pH. However, whether this holds true remains to be examined.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sequential pH culture (7.23 then 7.35) | Experimental | A split embryo at day 3 to continue culture at a pHe of 7.23±0.02 or to be cultured at a pH of 7.35±0.02 and monitor the effect on blastocyst development. |
|
| Continuously pH culture at 7.23 | No Intervention | Embryo culture from day 0 to 5 or 6 at 7.23 |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| pH Effect on blastocyst formation | Other | to investigate which pH is suitable for each stage of embryo development |
|
| Measure | Description | Time Frame |
|---|---|---|
| Ongoing Pregnancy rate | women with continued pregnancy at week 12 of gestation | 12 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| rate of usable blastocysts | number of blastocysts suitable for transfer on day 5 or 6 | 6 days of culture |
| Blastocyst formation rate | number of formed blastocyst per Fertilized oocyte |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Quena IVF Centre | Quena | Qena Governorate | 12346 | Egypt | ||
| Banon Assiut |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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| 6 days of culture |
| Clinical pregnancy rate | number of women with ultrasound detection of sacs with heartbeat per stimulated cycle | 3 months |
| Implantation rate | number of sacs with a heartbeat | 7 weeks |
| Asyut |
| Egypt |
| IbnSina IVF Center, IbnSina Hospital | Sohag | 12345 | Egypt |