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| Name | Class |
|---|---|
| The Research Council of Norway | OTHER |
| University of Oxford | OTHER |
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The aim of this study is to examine the effect of methionine and cysteine restriction on energy and macronutrient metabolism in overweight human subjects
The sulfur-containing amino acid methionine and its downstream metabolite cysteine has been associated with fat mass in animal studies and human observational studies. Some of the proposed mechanisms include an association with lipogenesis, insulin sensitivity and disrupted energy metabolism.
The overall aim of this project is to evaluate the effects of a diet low in methionine and cysteine on markers of lipids, amino acids, glucose and other biomarkers related to energy metabolism, including transcriptomic and protein expression data from adipose tissue and lymphocytes. Overweight and obese but otherwise healthy volunteers will be randomized to receive a diet either low, moderate or high in methionine and cysteine concentrations for 1 week.
This is a pilot study that may be considered an extension of NCT02647970. In addition to the primary and secondary outcomes we will evaluate the feasibility of the intervention and procedures.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Low met/cys diet | Experimental | Dietary intervention |
|
| Moderate met/cys diet | Experimental | Dietary intervention |
|
| High met/cys diet | Active Comparator | Dietary intervention |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Low met/cys diet | Behavioral | Dietary intervention with low contents of methionine and cysteine |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change in plasma concentrations of amino acids | Measurement of biomarkers of amino acid metabolism, including plasma concentrations of methionine, cysteine and related metabolites | Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Change in protein expression activity in tissues | Measurement of protein expression and activity related to methionine and cysteine metabolism in adipose tissue and lymphocytes. | Baseline and day 7 (final visit for adipose tissue, Baseline and day 1, 3 and 7 (final visit) for lymphocytes. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in plasma concentrations of fatty acids | Measurement of fatty acid content in plasma | Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Change in plasma activity indices of steaoryl-CoA desaturase |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Oslo | Oslo | 0372 | Norway |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 32160926 | Result | Olsen T, Ovrebo B, Haj-Yasein N, Lee S, Svendsen K, Hjorth M, Bastani NE, Norheim F, Drevon CA, Refsum H, Vinknes KJ. Effects of dietary methionine and cysteine restriction on plasma biomarkers, serum fibroblast growth factor 21, and adipose tissue gene expression in women with overweight or obesity: a double-blind randomized controlled pilot study. J Transl Med. 2020 Mar 11;18(1):122. doi: 10.1186/s12967-020-02288-x. | |
| 36542145 |
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| ID | Term |
|---|---|
| D050177 | Overweight |
| ID | Term |
|---|---|
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
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Participant: The diets are similar except for methionine and cysteine, and it is thought that the participants will not be able to identify their treatment allocation by taste.
Investigator: Randomization will be carried out by an investigator that is not related to the study. All foods will be pre-packaged and delivered to the participants by an unrelated company.
| Moderate met/cys diet | Behavioral | Dietary intervention with moderate contents of methionine and cysteine |
|
| High met/cys diet | Behavioral | Dietary intervention with high contents of methionine and cysteine |
|
Calculated based onthe ratio of plasma concentrations of products and precursors
| Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Change in protein expression of steaoryl CoA-desaturase in adipose tissue | Measurement of protein expression of steaoryl CoA-desaturase in adipose tissue | Baseline and Day 7 (final visit) |
| Change in protein expression of steaoryl CoA-desaturase in lymphocytes | Measurement of protein expression of steaoryl CoA-desaturase in lymphocytes | Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Change in steaoryl CoA-desaturase activity in adipose tissue | Measurement of enzyme activity of steaoryl CoA-desaturase in adipose tissue | Baseline and Day 7 (final visit) |
| Diet feasibility | Diet feasibility will be measured by a self-administered questionnaire | Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Diet compliance | Compliance with the diet will be measured by a self-administered questionnaire | Baseline, Day 1, Day 3 and Day 7 (final visit) |
| Body composition | Measurement of body composition by bioimpedance and dual X-ray absorptometry | Baseline and day 7 (final visit) |
| Derived |
| Elshorbagy A, Bastani NE, Lee-Odegard S, Ovrebo B, Haj-Yasein N, Svendsen K, Turner C, Refsum H, Vinknes KJ, Olsen T. The association of fasting plasma thiol fractions with body fat compartments, biomarker profile, and adipose tissue gene expression. Amino Acids. 2023 Mar;55(3):313-323. doi: 10.1007/s00726-022-03229-2. Epub 2022 Dec 21. |
| D012816 |
| Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |