Bioclinical Study of Scalp Photoaging of a Population Male Over 65 Years Old.

Bioclinical Study of Scalp Photoaging of a Population Male Over 65 Years Old. Mono-centric Prospective Study

The aging of skin, especially that of the scalp, is a combination of intrinsic and extrinsic aging factors secondary to the chronic sun exposure. For men with an alopecia genetic (= bald men), the solar part, in skin aging process, is major.

The helio-dermal scalp is frequently the site of cancers mainly after 65 years. Objectives are to identify specific biomarkers involved in the photo-aging of the alopecic scalp

Investigators can also hope to understand and to explain the inter-individual inequality of the aging of skin. They can hope to a better understanding of the biological mechanisms between photo-induced skin aging and photo-induced carcinogenesis.

Investigators propose to study 40 male patients aged 65 and over in order to compare the state of aging of their scalp skin in the alopecic zone :

not hairy photo-exposed (= ZH sun damage zone) versus occipital hair zone protected photo (= ZT control zone).

Patients will be recruited in the dermatology department of the St-Etienne University Hospital who will benefit from the removal of a scalp tumour in the alopecic zone.

This study aims to identify biological markers, by transcriptomic analysis, associated with aging of the scalp, by comparing for each patient the skin of the scalp exposed to the light ("ZH") to an unexposed witness skin ("ZT").

The study will include :

• examinations to characterize components of the scalp: transcriptomic, proteomic and immunohistochemical analysis of skin biopsies, and a swab sampling will identify the cutaneous microflora of the scalp
• noninvasive examinations, in vivo

Inclusion Criteria:

• Requiring excision of an alopecic scalp lesion
• from consent signed
• Patient affiliated or entitled to a social security.

Exclusion Criteria:

• scalp infection
• inflammatory disease of the scalp
• genetic disease with even partial expression of the scalp
• medical treatment of scalp less than 3 weeks old
• antecedent radiotherapy of the cephalic extremity
• prior surgery of the scalp with regard to the area sampled
• Application of hair implants, or any other surgical or medical procedures of the scalp
• Washing of the scalp or application of a cosmetic product for less than 48 hours
• Artificial hair coloring.
• any contraindication to surgical excision