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The aim of this research study is to characterize gut microbiota composition and activity after a daily supplementation of 4.5 g of chitin-glucan during 3 weeks. For this purpose, a monocentric longitudinal intervention study without control will be performed. Fecal microbiota composition and fecal concentrations of gut microbiota-derived metabolites (bile acids, conjugated-polyunsaturated fatty acids and short-chain fatty acids) will be measured. In addition, during ten hours following the ingestion of a test meal rich in fibers, the kinetic of production of H2, methane and volatile compounds exhaled from breath will be evaluated.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Chitin-glucan | Experimental | Supplementation during 3 weeks with 4.5g per day of chitin-glucan fiber |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Chitin-glucan fiber | Dietary Supplement | 4.5g per day of chitin-glucan during 3 weeks |
|
| Measure | Description | Time Frame |
|---|---|---|
| Gut microbiota composition | Illumina sequencing of 16SrDNA | Difference between day 0 and day 21 |
| Measure | Description | Time Frame |
|---|---|---|
| Fecal short-chain fatty acids | Gas chromatography using Flame ionization detector (GC- FID) | Difference between day 0 and day 21 |
| Fecal bile acids | Gas chromatography-mass spectrometry (GC/MS) |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Center of Investigation in Clinical Nutrition | Louvain-la-Neuve | 1348 | Belgium |
All data will be shared by the partners from the Consortium agreement.
At the end of the study
Belonging to the project Consortium
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Supplementation during 3 weeks with 4.5g of chitin-glucan
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| Difference between day 0 and day 21 |
| Fecal conjugated-linoleic acids | Gas chromatography using Flame ionization detector (GC- FID) | Difference between day 0 and day 21 |
| Exhaled acetate | Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled propionate | Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled butyrate | Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled C2 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled CO2 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled H2S | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled O2 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled N2 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled CH4 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled CO | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled H2 | Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Exhaled volatile compounds | Gas chromatography using selected ion flow tube mass spectrometry - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between day 0 and day 21 |
| Gastro-intestinal symptom - discomfort | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - nausea | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - bloating | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - flatulences | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - gastrointestinal reflux | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - cramps | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - rumbling | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - burps | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation | 3 weeks |
| Gastro-intestinal symptom - discomfort | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - nausea | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - bloating | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - flatulences | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - gastrointestinal reflux | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - cramps | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - rumbling | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |
| Gastro-intestinal symptom - burps | 100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion) | Difference between Day 0 and Day 21 |