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| Name | Class |
|---|---|
| Roquette Freres | INDUSTRY |
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The purpose of this study is to determine if supplementation with NUTRALYS pea protein isolate compared to whey protein and apple juice (carbohydrate, non-protein control) before, during, and after a 90-minute bout of eccentric exercise can attenuate exercise-induced muscle damage, inflammation, and delayed onset of muscle soreness (DOMS), and speed recovery of muscle function.
Study participants will be randomized to the NUTRALYS pea protein supplement, whey protein, or apple juice (parallel group design). Study participants will come to the lab at 7:00 am Monday through Friday in an overnight fasted state and provide a blood sample (maximum of 30 ml or two tablespoons). The blood samples will be analyzed for various measures of muscle damage and inflammation, and extra samples will be stored for additional measurements depending on funding.
After the blood draw, participants will provide a muscle soreness rating. Next, the two protein sources (pea and whey protein), will be consumed at a weight adjusted amount (0.3 grams of protein per kilogram of body weight), and administered under double blind procedures. The amount of apple juice will be adjusted to body weight (1.2 calories per kilogram body weight).
Four muscle function tests will be conducted: vertical jump, bench press, leg-back strength, and anaerobic power through the 30-second Wingate test. These performance tests will be administered before and after the 90-minute eccentric muscle exercise bout, and each of the following four mornings of recovery.
After taking the four performance tests, participants will engage in 90-minutes of eccentric exercise. Immediately following exercise, participants will provide a muscle soreness rating and a blood sample, ingest another protein dose (0.3 g/kg) (or apple juice), and then take the four muscle function tests. Participants will return at 7 am in an overnight fasted state four days in a row after the eccentric exercise bout, and provide muscle soreness ratings and blood samples followed by ingestion of the protein supplements or apple juice. Following ingestion of the supplements, participants will take the four muscle function tests.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Pea Protein | Experimental | NUTRALYS pea protein supplement |
|
| Whey Protein | Experimental | Whey protein supplement |
|
| Apple juice | Active Comparator | Apple juice |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| NUTRALYS pea protein supplement | Dietary Supplement | 0.3 grams of protein per kilogram of body weight before and after eccentric exercise, and each of 4 mornings of recovery |
|
| Measure | Description | Time Frame |
|---|---|---|
| Serum Creatine kinase | Muscle damage marker | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Measure | Description | Time Frame |
|---|---|---|
| Serum Myoglobin | Muscle damage marker | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Serum Lactate Dehydrogenase | Muscle damage marker |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| David C Nieman, DrPH | Appalachian State Univ | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Appalachian State University Human Performance Lab, North Carolina Research Campus | Kannapolis | North Carolina | 28081 | United States |
Depends on the journal where the paper will be published.
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| ID | Term |
|---|---|
| D007249 | Inflammation |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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| ID | Term |
|---|---|
| D000067816 | Whey Proteins |
| ID | Term |
|---|---|
| D008894 | Milk Proteins |
| D000080224 | Animal Proteins, Dietary |
| D004044 | Dietary Proteins |
| D011506 | Proteins |
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Randomized, parallel group
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| Whey Protein | Dietary Supplement | 0.3 grams of protein per kilogram of body weight before and after eccentric exercise, and each of 4 mornings of recovery |
|
| Apple juice | Dietary Supplement | 1.2 calories per kilogram body weight before and after eccentric exercise, and each of 4 mornings of recovery |
|
| Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Delayed onset of muscle soreness | Self-reported perception of muscle soreness, 1-10 scale | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Vertical jump | Muscle performance, vertical jump height | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Bench press | Muscle performance, bench press to exhaustion with weighted bar (75% body weight for men, 50% for women) | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Leg-back strength | Muscle performance, leg-back strength with a dynamometer | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| 30-second Wingate cycle test | Anaerobic power cycling test, peak and average anaerobic power | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Serum C-reactive protein | Inflammation measurement | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| Plasma cytokine panel | IL-6, IL-8, IL-10, MCP-1, IL-1ra, GCSF | Change from pre-exercise to post-exercise (immediately after, and 1-, 2-, 3-, and 4-days after) |
| D000602 |
| Amino Acids, Peptides, and Proteins |
| D000067796 | Whey |
| D008892 | Milk |
| D003611 | Dairy Products |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D019602 | Food and Beverages |