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The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.
Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 'normal' abstinence duration | Active Comparator | Patients follow the normal abstinence duration as asked by the physician (2-7 days). This sperm will be used to inject half of the oocytes Intervention = second sperm sample |
|
| short abstinence duration | Experimental | Patients will be asked to produce a second sperm sample, 2 hours after the first. This sperm will be used to inject the other half of the oocytes. Intervention = second sperm sample |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| second masturbation | Other | Husbands will be asked to produce a second sperm sample in the masturbatorium |
|
| Measure | Description | Time Frame |
|---|---|---|
| Embryo development (blastocyst stage) | embryo development 5/6 of the preimplantation development | 1 year |
| Embryo development (cleavage stage) | embryo development on day 3 of the preimplantation development | 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Fertilization | number of fertilized oocytes per number of mature oocytes | 1 year |
| Utilization rate | number of embryos used for transfer + cryopreserved for the patient |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Neelke De Munck, PhD | Universitair Ziekenhuis Brussel | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universitair Ziekenhuis Brussel | Brussels | Brussels Capital | 1090 | Belgium |
No data available for researchers others than the ones involved in the study
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a prospective sibling oocyte study: both study arms will be applied to the oocytes of 1 patient
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| 1 year |
| Sperm quality | concentration and motility | 1 year |
| Sperm DNA fragmentation | analysis of non-viable sperm in the two samples | 1 year |
| Clinical Pregnancies | pregnancies with a fetal heart beat | 1 year |