DNA Methylation and 1-C Metabolism Nutrient Intake

Dietary Intake of One-carbon Metabolism Nutrients and DNA Methylation in Peripheral Blood

The present study used a sub-sample of the Melbourne Collaborative Cohort Study (MCCS) (Milne RL et al, Int J Epidemiol, 2017), who had valid data on dietary intakes (121-item food frequency questionnaire) and valid measures of DNA methylation using the HumanMethylation Infinium 450K Beadchip assay, or using LINE-1, Alu and Sat2 assays.

The investigators studied the association between nutrients involved in one-carbon metabolism and DNA methylation in peripheral blood.

Inclusion Criteria:

• Selected into one of six case-control studies nested in the Melbourne Collaborative Cohort Study
• Completed a 121-item Food Frequency Questionnaire
• Had a blood DNA sample with a measure of methylation

Exclusion Criteria:

• Missing data in any of the outcome, exposure, or confounders (N=797 excluded)