Gingival Crevicular Fluid Levels of Protein Carbonyl Following the Use of Lycopene in Chronic Periodontitis

Gingival Crevicular Fluid Levels of Protein Carbonyl Oxidative Stress Biomarker Following the Use of Locally Delivered Lycopene Antioxidants in Patients With Chronic Periodontitis - Randomized Clinical Trial

Effect of Locally Delivered Antioxidants as An Adjunct to Non-surgical Periodontal Therapy on GCF Level of Oxidative Stress Marker; Protein Carbonyl

The study was designed as a randomized controlled clinical trial. Total subjects selected were allocated into three study groups, Group I (Test group), Group II (Positive control group) and Group III (Negative control group). Group I: Included 8 patients with severe chronic periodontitis managed by Scaling and root planing and local delivery of lycopene loaded in solid lipid microparticles. Group II: Included 8 patients with severe chronic periodontitis managed by Scaling and root planing only. Group III: Included 8 patients with clinically healthy periodontium.

Protein carbonyl biomarker level and clinical parameters were assisted to evaluate the effect of lycopene as an adjunct to scaling and root planing.

Inclusion Criteria:

• All subjects were free from any systemic diseases.
• Subjects for group I and II were suffering from severe chronic periodontitis (Armitage et al., 1999).
• Group III included subjects with clinically healthy periodontium.
• All subjects were between 35-60 years old.
• Both genders were included.
• Subjects were able to return for follow up visits.
• Subjects agreed to sign a written consent after the nature of the study was explained.

Exclusion Criteria:

• History of scaling and root planing or periodontal surgery < 6 months prior to initiation of the study.
• History of administration of antimicrobial drugs or over the counter antioxidants like Vit C, Vit B, β-carotene < 6 months prior to the baseline examination.
• Subjects who reported any side effects or drug allergies from antioxidants.
• Subjects with mobile teeth, abscesses and defected restorations related to the affected sites.
• Subjects with para-functional habits.
• Pregnant and lactating females.
• Smokers, alcoholic and drug abusers.
• Prisoner.
• Mentally retarded patients.