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| Name | Class |
|---|---|
| Newcastle University | OTHER |
| University of Aberdeen | OTHER |
| Aix Marseille Université | OTHER |
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This study investigates the effect of genetic variations after supplementation with Brazil nuts in healthy Brazilians. Briefly, all the participants will consume one nut a day for 2 months and will stop the intake for more 2 months. Five blood sampling collection will be performed in one month interval, starting at baseline and ending at 2 months without intervention.
Brazil nuts are the richest source of selenium in nature, a micronutrient that has essential functions in human physiology.
Selenium status can be measured by the quantification of biomarkers in the blood. Previous studies have shown that genetic polymorphisms can affect Se status and modulate risk for chronic diseases. In this study, five biomarkers will be quantified in the blood, before and after supplementation with Brazil nuts. The genetic variants will be used to stratify the biomarkers and multivariate linear regressions will be used to explore the influence of the polymorphisms on concentrations of the biomarkers .
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Brazil Nut supplementation | Experimental | Consumption of one Brazil nut a day for 2 months followed by 2 months without intervention |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Brazil nut | Dietary Supplement | one nut a day for 2 months, no intervention for 2 months |
|
| Measure | Description | Time Frame |
|---|---|---|
| change in Plasma Se concentrations | measured by ICP-MS (ug/L) | 4 weeks |
| change in Plasma Glutathione Peroxidase 3 activity | measured by kinetic assay in a spectrometer (U/L) | 4 weeks |
| change in Plasma Selenoprotein P concentrations | measured by ELISA (mg/dL) | 4 weeks |
| change in Erythrocyte Se concentrations | measured by ICP-MS (ug/L) | 4 weeks |
| change in Erythrocyte Glutathione Peroxidase 1 activity | measured by kinetic assay in an automatic spectrometer (U/gHb) | 4 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| change in total cholesterol concentration in serum | measured in an automatic spectrometer (mg/dL) | 4 weeks |
| change in tryglicerides concentration in serum | measured in an automatic spectrometer (mg/dL) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Silvia MF Cozzolino, PhD | University of Sao Paulo | Principal Investigator |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 27164132 | Result | Donadio JL, Guerra-Shinohara EM, Rogero MM, Cozzolino SM. Influence of Gender and SNPs in GPX1 Gene on Biomarkers of Selenium Status in Healthy Brazilians. Nutrients. 2016 May 5;8(5):81. doi: 10.3390/nu8050081. | |
| 28501922 | Result | Donadio JLS, Rogero MM, Guerra-Shinohara EM, Desmarchelier C, Borel P, Cozzolino SMF. SEPP1 polymorphisms modulate serum glucose and lipid response to Brazil nut supplementation. Eur J Nutr. 2018 Aug;57(5):1873-1882. doi: 10.1007/s00394-017-1470-7. Epub 2017 May 13. |
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| ID | Term |
|---|---|
| C103364 | 2S albumin, brazil nut |
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| 4 weeks |
| change in HDL concentration in serum | measured in an automatic spectrometer (mg/dL) | 4 weeks |
| change in LDL concentration in serum | measured in an automatic spectrometer (mg/dL) | 4 weeks |
| change in protein-c reactive concentration in serum | measured in an automatic spectrometer (mg/dL) | 4 weeks |
| change in Plasma MDA concentrations (malondialdehyde) | measured in an automatic spectrometer (mg/dL) | 4 weeks |
| 28696394 | Result | Donadio JLS, Rogero MM, Cockell S, Hesketh J, Cozzolino SMF. Influence of Genetic Variations in Selenoprotein Genes on the Pattern of Gene Expression after Supplementation with Brazil Nuts. Nutrients. 2017 Jul 11;9(7):739. doi: 10.3390/nu9070739. |
| 29609868 | Result | Donadio JLS, Rogero MM, Guerra-Shinohara EM, Barbosa F Jr, Desmarchelier C, Borel P, Sneddon AA, Hesketh JE, Cozzolino SMF. Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study). Clin Nutr. 2019 Apr;38(2):539-548. doi: 10.1016/j.clnu.2018.03.011. Epub 2018 Mar 23. |