Myo-inositol/D-chiro-inositol Ratio in Follicular Fluid

Altered Myo-inositol/D-chiro-inositol Ratio in Follicular Fluid of Women Undergoing in Vitro Fertilization Has Detrimental Effects on Oocyte and Embryo Quality

Considering the importance assumed by follicular microenvironment for a proper oocyte development, this study is a prospective observational clinical trial which aims to evaluate whether the embryo blastocyst quality may be affected by different Myo-inositol (MI) and D-chiro-inositol (DCI) levels in the follicular fluid (FF) of the aspired oocytes. The study includes egg donors and egg receiving couples participating in the oocyte donation program. MI/DCI is calculated in FF and related with blastocyst grade.

Inclusion Criteria:

• clear follicular aspirate obtained during oocyte retrieval;
• each specimen of follicular fluid containing only one oocyte.

Exclusion Criteria:

• hyperglycaemic women;
• PCOS women also if euglycaemic;
• diabetic subjects having low concentrations of detectable MI in association with an anomalous MI transport.