Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays

For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control.

Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation).

Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health.

After extraction space closure, results will be assessed by clinical effects with biological markers levels:

CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ

Those biomarkers levels will be performed using ELISA Assays. After collecting data, the results will be statistically analyzed using Shapiro-Wilk and, posteriorly, T-student or Wilcoxon.

Inclusion Criteria:

• Adult subjects ages 18-40 years old, in good general health, with adult complete dentition, regardless of presence of third molars.
• Indication for orthognathic surgery
• Tooth extraction indication for orthodontic treatment
• Do not smoke
• Periodontal and teeth healthy
• Do not use bisphosphonates or corticosteroids

Exclusion Criteria:

• Surgically assisted rapid palatal expansion indication
• Cleft lip and palate
• Craniofacial syndrome
• Mental disorder
• Metabolic diseases