Tachykinin and Kisspeptin Expression in Human Granulosa and Cumulus Cells

Development of a New Diagnostic Tool to Assess Oocyte Quality Based on Tachykinin and Kisspeptin Expression Analysis in Human Granulosa and Cumulus Cells

The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.

Two groups of subjects (each one with different criteria):

1. Oocyte donors

   Inclusion Criteria:

   • Age between 18 and 34 years
   • Normal caryotype
   • Normal psychological test

   Exclusion Criteria:

   • Presence of hereditary diseases
   • Beta-thalassemia
   • Cystic fibrosis
   • Human immunodeficiency virus (HIV), Hepatitis B virus (HBV), Hepatitis C virus (HCV)

2. Infertility patients

Inclusion Criteria:

• Age between 18 and 45 years
• One of the following etiologies:
• Patient with PCOS according to Rotterdam Criteria or
• Patients with endometriosis stage I-IV or
• Patients with low ovarian response according to Bologna criteria or
• Patients with advances maternal age (between 38 and 45 years)