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The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Donors | Active Comparator | Oocyte donors without infertility problems |
|
| PCOS | Experimental | Patients with Polycystic Ovary Syndrome (PCOS) according to Rotterdam criteria |
|
| Endometriosis | Experimental | Patients with endometriosis (I-IV) |
|
| Age | Experimental | Patients with advanced maternal age (38 years or more) |
|
| Low ovarian response | Experimental | Patients with low ovarian response according to Bologna criteria |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Ovarian puncture | Procedure | Donors and patients are subjected to ovarian puncture as part of their donation procedure or IVF treatment. Oocyte are retrieved in this procedure along with granulosa and cumulus cells. Donors and patients sign an informed consent in order to donate their granulosa and cumulus cells for the study. |
| Measure | Description | Time Frame |
|---|---|---|
| Expression level of Substance P (SP) | Expression analysis at mRNA (mRNA = messenger ribonucleic acid) and protein level using techniques of real time quantitative PCR (PCR = polymerase chain reaction), immunocytochemistry and western blot | 4 years |
| Expression level of neurokinin A (NKA) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression level of neurokinin B (NKB) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression level of hemokinin 1 (HK-1) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression level of neurokinin 1 receptor (NK1R) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression of neurokinin 2 receptor (NK2R) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression level of neurokinin 3 receptor (NK3R) |
| Measure | Description | Time Frame |
|---|---|---|
| Patient or donor age | Years | 4 years |
| Patient or donor body mass index | kg/m2 | 4 years |
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Two groups of subjects (each one with different criteria):
Oocyte donors
Inclusion Criteria:
Exclusion Criteria:
Infertility patients
Inclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Instituto de Investigaciones Químicas | Seville | 41011 | Spain | |||
| IVI Sevilla |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 25316443 | Background | Garcia-Ortega J, Pinto FM, Fernandez-Sanchez M, Prados N, Cejudo-Roman A, Almeida TA, Hernandez M, Romero M, Tena-Sempere M, Candenas L. Expression of neurokinin B/NK3 receptor and kisspeptin/KISS1 receptor in human granulosa cells. Hum Reprod. 2014 Dec;29(12):2736-46. doi: 10.1093/humrep/deu247. Epub 2014 Oct 14. | |
| 27146034 | Result |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
| 4 years |
| Expression level of kisspeptin 1 (KISS1) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Expression level of kisspeptin 1 receptor (KISS1R) | Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot | 4 years |
| Years of infertility | Number of years that the patient has been trying to have a child without success | 4 years |
| Estradiol level (day of ovulation induction) | Estradiol level in blood (pg/mL), measured the day in which ovulation is induced | 4 years |
| Progesterone level (day of ovulation induction) | Progesterone level in blood (ng/mL), measured the day in which ovulation is induced | 4 years |
| Number of oocytes retrieved in the ovarian puncture | 4 years |
| Number of mature oocytes (metaphase II) obtained in the ovarian puncture | 4 years |
| Fertilization rate | Proportion of oocytes correctly fertilized (2 pronuclei and 2 polar bodies) after the ICSI (ICSI = intracytoplasmic sperm injection) procedure | 4 years |
| Embryo quality | Embryo quality at day 3 of embryo development (of each embryo of the cohort). Quality assessed according to ASEBIR (ASEBIR = "Asociación Española para el estudio de la Biología de la Reproducción" = Spanish society for reproductive biology) criteria. Embryo quality grade A to D. | 4 years |
| Daily and total dose of FSH (FSH = follicle stimulating hormone) | Daily dose of FSH for controlled ovarian stimulation. International Units (IU) | 4 years |
| Daily and total dose of HMG (HMG = human menopausal gonadotropin) | Daily dose of HMG for controlled ovarian stimulation. International Units (IU) | 4 years |
| Type of ovulation induction | Ovulation induction using 6500 IU of hCG (hCG = human chorionic gonadotropin) or 0.2 mg of triptorelin (Decapeptyl) | 4 years |
| Seville |
| 41011 |
| Spain |
| Garcia-Ortega J, Pinto FM, Prados N, Bello AR, Almeida TA, Fernandez-Sanchez M, Candenas L. Expression of Tachykinins and Tachykinin Receptors and Interaction with Kisspeptin in Human Granulosa and Cumulus Cells. Biol Reprod. 2016 Jun;94(6):124. doi: 10.1095/biolreprod.116.139881. Epub 2016 May 4. |