Evaluation of Granulosa Cells Function After IVF Protocols

In Vitro Evaluation of Luteinized Granulosa Cells After Being Submitted to in Vitro Fertilization Protocols With Gonadotropin-Releasing Hormone (GnRH) Agonist and GnRH Antagonist

This study is intended to evaluate ovarian cells after being submitted do different in vitro fertilization protocols. The investigators will analyze hormones secretion and intracellular mechanisms of these cells comparing the GnRH agonist protocol and the GnRH antagonist protocol.

Inclusion Criteria:

• Patient age limit: 39 years
• Follicle Stimulating Hormone (FSH) < 10 micrometer (mU) / Liter
• Antral follicular count > 12
• Regular menses (25 to 35 days)
• More than 3 oocytes after ovarian punction
• Anti Mullerian between 1 e 3 ng/ml

Exclusion Criteria:

• Patients submitted to intracytoplasmatic sperm injection (ICSI)
• Ovarian mass or cyst
• Clinical / endocrine pathologies