Sexual Steroids: Relationship Between Serum and Prostatic Tissue Level

Patients followed in the Foch Hospital Urology Department (Suresnes): Patients justifying a prostatectomy.

• Patients justifying prostatectomy together with the bladder (radical cystectomy for bladder cancer).
• Patients with benign prostate hyperplasia who justified a prostatectomy. Compare serum sexual steroid concentrations and intra-tissue on healthy prostates and prostate adenoma, assess concentrations intra-tissue sex steroids on cancer metastasis prostate specific blood sample under study (30mL) will be performed preoperatively in Patients followed in Foch Hospital Urology Department (Suresnes), and a Removal of a fragment of prostate tissue or metastasis will be analyze.

Aim is to compare serum concentrations of sexual steroids and intra-tissue on healthy prostates and prostate adenomas compared to concentrations measured in patients operated for prostate cancer.

Steroids concentration will be determined using Gas chromatography-mass spectrometry. The analyzed steroids are :

FSH :Mul/ml LH: mUI/ml SHBG: µg/ml BT: ng/ml TT: ng/ml DHT: ng/ml DHEA: ng/ml D5: ng/ml D4: ng/ml E1: pg/ml E2 : pg/ml DHEA-S : µg/dl

Inclusion Criteria:

• Men aged over 18 years
• Surgical indication justifying protatique prostatectomy, or complete prostatocystectomie, or removal of a metastasis of prostate cancer.
• Patients affiliated to a social security scheme or benefiting from such a regime.
• Patients who have given their consent to participate in writing

Exclusion Criteria:

• Patient received local therapy or hormonal treatment (LHRH analogue, therapy blocking androgen receptors) before surgery.
• Treatment reductase inhibitors 5ARI ongoing or stopped for less than 3 months before the intervention.
• Patient included in another clinical trial inconsistent with the conduct of this research.
• Patient receiving treatment that could interfere with hormone levels (Prednisone, Ketoconazole, Abiraterone, Finasteride, Dutasteride).