Comparison of Biofilm Characteristics Between an in Situ Biofilm Model of Supragingival Plaque and Natural Teeth

Comparison of Biofilm Characteristics Between an in Situ Biofilm Model of Supragingival Plaque and Natural Teeth

The purpose of this study is to determine in situ the bacterial viability, thickness and bacterial diversity of a biofilm formed on different substrates in comparison to a naturally tooth-formed oral biofilm. In addition, it will be evaluated whether the restraint of oral hygiene measures may influence the development of the PL-Biofilm.

• To calculate an a priori sample size, the following statistical criteria were established: an effect size of 0.35, an alpha error of 0.05 and a statistical power of 87%. Assuming these criteria and using the repeated measures ANOVA test, a sample size of 15 subjects was required.
• Bacterial viability and thickness will be analyse by Confocal Laser Scanning Microscope and Live/Dead BackLight staining solution.
• Bacterial diversity will be analyse by Pyrosequencing the 16S DNAr gene.

Inclusion Criteria:

• systemically healthy
• good oral health status

Exclusion Criteria:

• smoker or former smoker,
• presence of dental prostheses or orthodontic devices,
• antibiotic treatment or routine use of oral antiseptics in the previous 3 months,
• presence of any systemic disease that could alter the production or composition of saliva