Investigation of Bacterial Adhesion and Bactericide Effect ex Vivo on Different Implant Restoration Materials

Investigation of Bacterial Adhesion and Bactericide Effect ex Vivo on Different Implant Restoration Materials

Investigation of bacterial adhesion and bactericide effect ex vivo on different implant restoration materials

The aim of this study is to investigate bacterial attachment and bactericidal effects of different materials used for the transmucosal part of implants. Using a brace, the intraoral biofilm formation in healthy subjects will be measured after 28 hours (24 hours intraorally, 4 hours rest). For this purpose 2 sterile platelets of each of the 8 differently processed materials (16 platelets in total) are inserted into the braces.

The main target parameter is the quantification of the biofilm by crystal violet staining. For this purpose the samples are analyzed by spectrophotometry at a wavelength of 595 nm (OD595).

As secondary target parameter possible bactericidal effects of the materials, which are analyzed by Colony forming units (CFU) determination, are used.

Inclusion Criteria:

• No systemic antibiotic therapy during the last 3 months
• Good oral hygiene and compliance (plaque index (PI) <20%, bleeding index (BI) <20%)
• No signs of periodontitis or other inflammatory changes in surrounding tissues
• Non-smokers

Exclusion Criteria:

• Disease that requires antibiotics
• Pressure points caused by the braces, which cannot be eliminated by adjusting
• Other unforeseen complications that can be associated with braces