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There are no reports involved the intestinal microbiota from Chinese infants with short bowel syndrome (SBS) under different clinical status. Alterations in the microbiota are closely correlated with the bile acids and short chain fatty acids metabolism as well as the intestinal immunity. A relatively comprehensive profile composed of microbial structure, microbial metabolism products and immune biomarkers in SBS infants may facilitate a better therapy strategy to complications occurred in SBS children.
The investigators totally collected 26 fecal samples from 18 infants diagnosed with SBS during parenteral nutrition administration, and these samples were divided into three groups according to complications of enrolled patients at sampling time: asymptomatic group, central catheter-related blood stream infections group and liver injury group. 7 healthy infants with supplementary food were enrolled as control. Fecal microbiota, sIgA and calprotectin, bile acids and short chain fatty acids were also detected by 16S ribosomal ribonucleic acid (rRNA) gene sequencing, enzyme-linked immunosorbent assay and liquid/gas chromatography.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| "Asymptomatic" | "Asymptomatic" meaning patients showed well tolerance to parenteral nutrient (PN) administration and there were no complications occurred within two months (n=7); |
| |
| CLABSI | with central catheter-related blood stream infections (CLABSI) meaning patients had fever, increased neutrophils, documented positive catheter blood culture but exclude other source of infection (n=5) |
| |
| PNALD | with parenteral nutrient associated liver disease (PNALD), meaning SBS patients showed elevated liver enzymes and bilirubin (n=14). |
| |
| healthy controls | Seven healthy infants who had added complementary were served as controls (n=7). |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Complications | Other | Not involved |
|
| Measure | Description | Time Frame |
|---|---|---|
| Microbial structure in SBS infants | Fecal microbiota were detected by 16S rRNA gene sequencing. | up to 4 months |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolism products in SBS infants | Bile acids and short chain fatty acids were detected by liquid/gas chromatography. | up to 4 months |
| Immune biomarkers in SBS infants | Secretary IgA and calprotectin were detected by enzyme-linked immunosorbent assay. |
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Inclusion Criteria:
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18 infants diagnosed with SBS were enrolled from Digestion and Nutrition Division at Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine.
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| Name | Affiliation | Role |
|---|---|---|
| Guangyu Chen, PhD | Shanghai Municipal Science and Technology Commission | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ethics Committee of Xinhua Hospital | Shanghai | Shanghai Municipality | 200092 | China |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
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| ID | Term |
|---|---|
| D012778 | Short Bowel Syndrome |
| ID | Term |
|---|---|
| D008286 | Malabsorption Syndromes |
| D007410 | Intestinal Diseases |
| D005767 | Gastrointestinal Diseases |
| D004066 | Digestive System Diseases |
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| D011183 | Postoperative Complications |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |