Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Ministry of Education, Malaysia | OTHER_GOV |
| Universiti Malaysia Pahang | OTHER |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The project is a randomized, 3-way, blinded crossover trial in which 20 healthy, fasted participants consume meals with 30 g of a linear corn starch and 5 g of one of the seaweeds, Laminara digitata or Undaria pinnatifida or a pea protein control. The primary aim is to investigate whether the brown seaweeds affect the postprandial glucose Area Under the Curve (AUC). Stomach emptying, insulin, C-peptide, appetite-regulating hormones (oxyntomodulin, glucagon, GLP-1 and PYY), and specific metabolites from the seaweeds in the urine and plasma as well as subjective satiety are also analyzed.
Brown seaweeds may contain bioactive compounds as they contain secondary plant metabolites, including flavonoids and other phenolic compounds as well as carotenoids. Dietary fibre is the major component of brown seaweeds, mainly composed from laminarans, alginates, fucans and cellulose. Several dietary fibers and secondary plant metabolites have been shown to have an impact on health and more specifically on glycemic control. Therefore, the aim of this study was to investigate whether two selected brown seaweeds have an effect on the postprandial glucose response to a starch load as well as several secondary measures, including appetite responses.
In a controlled crossover trial, 20 healthy fasted participants consumed 5 g of either Laminaria digitata (LD) or Undaria pinnatifida (UP) or 1 g of pea protein (CTR). The volunteers concomitantly ingested 200 mL of a drink with corn starch. Blood samples were drawn at baseline, as well as 20, 40, 60, 90, 120 and 180 min after the meal. Differences in glucose, insulin, C-peptide, plasma incretin concentrations, and insulin sensitivity index (ISI) were analyzed and appetite was scored by a visual analogue scale and a subsequent ad libitum test meal.
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sequence A | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence LD-UP-CTR |
|
| Sequence B | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence LD-CTR-UP |
|
| Sequence C | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence UP-LD-CTR |
|
| Sequence D | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence UP-CTR-LD |
|
| Sequence E | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence CTR-LD-UP |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| LD | Other | Participants ingested 5 g of Laminaria digitata followed by 30 g of corn starch mixed with 200 mL of water. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Plasma glucose Area Under the Curve (Glucose AUC) | The area under the plasma glucose concentration curve was calculated from 0-180 min after 30 g of corn starch was ingested. | 0- 180 min |
| Measure | Description | Time Frame |
|---|---|---|
| Plasma glucose concentrations | Changes in the glucose concentrations determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate. | 20, 40, 60, 90, 120 and 180 min |
| Serum Insulin Area Under the Curve |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic profile in urine | Untargeted metabolic profile of urine samples measured in all samples collected before the meal and postprandially from 0-90 min, 90-180 min as well as in samples collected up to 24 hours later. The totality of the profiles is used to explore for changes after baseline as detected by multivariate statistics (PLS-DA) after dividing the data into a training and test set. Contrasts between each of the treatment groups and the placebo group will be assessed using the receiver-operator characteristics (ROC curves) comparing the training and test set. |
Inclusion Criteria:
BMI 20-25 kg/m2. Waist circumference < 102 cm. Non-smoking. Like (can tolerate) seaweed, pasta, meat sauce as these foods are part of the test meal.
Can tolerate paracetamol (Pinex, Actavis, Denmark).
Exclusion Criteria:
Systemic infections, psychiatric or metabolic diseases, and any clinical condition, chronic or frequent use of medication (including blood thinners, excluding contraceptives), smoking (throughout the trial and 12 months before the start of the experiment), blood donations during or in the month leading up to the study period, elite athletes (> 10 hours of hard exercise / week, self-reported), high intake of alcohol (defined as a weekly intake of > 7 units for women and > 14 units for men), have or have had a drug addiction, participation in other scientific studies during the study period, lactating, pregnancy or ongoing planning of pregnancy, and vegetarianism or veganism.
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Lars O Dragsted, PhD | University of Copenhagen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Nutrition, Exercise and Sports, University of Copenhagen | Frederiksberg | 1958 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 32917417 | Derived | Zaharudin N, Tullin M, Pekmez CT, Sloth JJ, Rasmussen RR, Dragsted LO. Effects of brown seaweeds on postprandial glucose, insulin and appetite in humans - A randomized, 3-way, blinded, cross-over meal study. Clin Nutr. 2021 Mar;40(3):830-838. doi: 10.1016/j.clnu.2020.08.027. Epub 2020 Aug 31. |
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D006943 | Hyperglycemia |
| D007333 | Insulin Resistance |
| ID | Term |
|---|---|
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D006946 | Hyperinsulinism |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
|
| Sequence F | Experimental | The meal study was performed with Laminaria digitata (LD), Undaria pinnatifida (UP) or pea protein drink (CTR) in the sequence CTR-UP-LD |
|
| UP | Other | Participants ingested 5 g of Undaria pinnatifida followed by 30 g of corn starch mixed with 200 mL of water. |
|
| CTR | Other | Participants ingested 30 g of corn starch and 1 g of pea protein powder mixed with 200 mL of water. |
|
Area Under the Curve (AUC) for serum insulin.
| 0-180 min |
| Serum Insulin concentrations | Changes in the insulin concentrations determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate. | : 20, 40, 60, 90, 120 and 180 min |
| ISI (0-180) | Matsuda's Insulin Sensitivity Index | 0-180 min |
| Serum C-peptide Area Under the Curve | Area Under the Curve (AUC) for serum C-peptide. | 0-180 min |
| Serum C-peptide concentrations | Changes in the C-peptide concentrations determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate. | 20, 40, 60, 90, 120 and 180 min |
| Plasma GLP-1 Area Under the Curve | Area Under the Curve (AUC) for plasma GLP-1. | 0-180 min |
| Plasma GLP-1 concentrations | Changes in the plasma GLP-1 concentrations determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate | 20, 40, 60, 90, 120 and 180 min |
| Subjective appetite scores assessed by visual analogue scale (VAS) | Area Under the Curve (AUC) for all VAS outcomes (satiety, hunger, fullness, prospective food consumption and comfort) | 0-180 min |
| Subjective appetite scores assessed by visual analogue scale (VAS) | Changes in VAS outcomes (satiety, hunger, fullness, prospective food consumption and comfort), determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate | 20, 40, 50, 70, 100, 130 and 180 min |
| 0-24 hrs |
| Metabolic profile in plasma | Untargeted metabolic profile of plasma measured in all samples collected before the meal and postprandially from 0-180 min. The totality of the profile is used to explore for changes after baseline as detected by multivariate statistics (PLS-DA) after dividing the data into a training and test set. Contrasts between each of the treatment groups and the placebo group will be assessed using the receiver-operator characteristics (ROC curves) comparing the training and test set. | 0-180 min |