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| Name | Class |
|---|---|
| The Scripps Research Institute | OTHER |
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A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.
BACKGROUND:
A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.
OBJECTIVES:
To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.
DESIGN AND METHODOLOGY:
Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Water | Placebo Comparator | The subjects will ingest the HFHC meal with 500mL of water. |
|
| Orange juice | Experimental | The subjects will ingest the HFHC meal with 500mL of orange juice. |
|
| Water with glucose | Active Comparator | The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice). |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Orange juice | Other | Individuals will consume the HFHC meal with 500mL of orange juice. |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry | before the meal, 1h, 3h and 5h after the meal |
| Measure | Description | Time Frame |
|---|---|---|
| Blood glucose | before the meal | |
| Blood glucose | 1h after the meal | |
| Blood glucose |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Daniela FS Chaves, PhD | University of Sao Paulo | Principal Investigator |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 20200256 | Result | Ghanim H, Sia CL, Upadhyay M, Korzeniewski K, Viswanathan P, Abuaysheh S, Mohanty P, Dandona P. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010 Apr;91(4):940-9. doi: 10.3945/ajcn.2009.28584. Epub 2010 Mar 3. | |
| 23417730 |
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| ID | Term |
|---|---|
| D007249 | Inflammation |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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| ID | Term |
|---|---|
| D014867 | Water |
| D005947 | Glucose |
| ID | Term |
|---|---|
| D006878 | Hydroxides |
| D000468 | Alkalies |
| D007287 | Inorganic Chemicals |
| D000838 | Anions |
| D007477 |
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| Water |
| Other |
Individuals will consume the HFHC meal with 500mL of water. |
|
| Water with glucose | Other | Individuals will consume the HFHC meal with 500mL of water with glucose. |
|
| 3h after the meal |
| Blood glucose | 5h after the meal |
| Insulin | before the meal |
| Insulin | 1h after the meal |
| Insulin | 3h after the meal |
| Insulin | 5h after the meal |
| Lipid profile | total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides | before the meal |
| Lipid profile | total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides | 1h after the meal |
| Lipid profile | total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides | 3h after the meal |
| Lipid profile | total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides | 5h after the meal |
| Coelho RC, Hermsdorff HH, Bressan J. Anti-inflammatory properties of orange juice: possible favorable molecular and metabolic effects. Plant Foods Hum Nutr. 2013 Mar;68(1):1-10. doi: 10.1007/s11130-013-0343-3. |
| 28927270 | Derived | Chaves DFS, Carvalho PC, Brasili E, Rogero MM, Hassimotto NA, Diedrich JK, Moresco JJ, Yates JR 3rd, Lajolo FM. Proteomic Analysis of Peripheral Blood Mononuclear Cells after a High-Fat, High-Carbohydrate Meal with Orange Juice. J Proteome Res. 2017 Nov 3;16(11):4086-4092. doi: 10.1021/acs.jproteome.7b00476. |
| Ions |
| D004573 | Electrolytes |
| D010087 | Oxides |
| D017601 | Oxygen Compounds |
| D006601 | Hexoses |
| D009005 | Monosaccharides |
| D000073893 | Sugars |
| D002241 | Carbohydrates |