Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The purpose of this study is to analyze the influence of polymorphisms of the genes CLDN-1 (Claudina-1), LGALS3 (Lectin galactoside-binding soluble 3), SOCS3 (Suppressor of cytokine signaling 3), IL-28B (interleukin-28B), CCL5 (Chemokine C-C ligand 5) in the determination of clinical forms and in the percentage of cardiac fibrosis in patients with Chagas disease.
The Chagas disease, in its chronic phase, can lead to the development of cardiac arrhythmias and dilated cardiomyopathy, which can evolve to heart failure, causing great morbidity and reduction on patient's quality of life. Some patients stay asymptomatic while others develop the cardiac form of the disease (chronic chagasic cardiomyopathy), or other forms, like the digestive (megacolon and megaesophagus) and the mixed form (cardiac and digestive).
The objective of this study is to analyze the association between single nucleotide polymorphisms with the clinical forms and the amount of cardiac fibrosis in patients with Chagas disease.
The polymorphisms will be analyzed by real time PCR (Polymerase Chain Reaction). The genes whose polymorphisms will be analyzed are: galectin-3, SOCS-3, IL-28B, CLDN-1 and CCL5.
The patients shall be accompanied in the specialized outpatient clinics for Chagas disease - Center of Biotechnology and Cell Therapy. They will be submitted to collection of blood samples for biochemical analysis and cardiac magnetic resonance imaging.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Single group | Group which contains all the patients of the study. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Gathering of samples of genetic material | Genetic | The samples will be obtained from peripheral blood mononuclear cells and the genotyping of the polymorphisms will be done by real time PCR. |
| Measure | Description | Time Frame |
|---|---|---|
| Presence of polymorphisms of the genes CLDN-1, LGALS3, SOCS3, IL-28B, CCL5 in the different forms of Chagas disease | We will evaluate the presence of polymorphisms of the genes CLDN-1, LGALS3, SOCS3, IL-28B, CCL5 in the different forms of Chagas disease. | The outcome measure will be assessed within one month of follow up. |
| Measure | Description | Time Frame |
|---|---|---|
| Presence of polymorphisms of the genes CLDN-1, LGALS3, SOCS3, IL-28B, CCL5 in subjects with and without myocardial fibrosis | We will evaluate the presence of polymorphisms of the genes CLDN-1, LGALS3, SOCS3, IL-28B, CCL5 in subjects with and without myocardial fibrosis assessed by magnetic resonance. | The outcome measure will be assessed within one month of follow up. |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Tertiary Hospital
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Milena BP Soares, PhD | Hospital São Rafael | Principal Investigator |
| Ricardo R dos Santos, PhD | Hospital São Rafael | Study Director |
| Bruno SF Souza, MD, Msc | Hospital São Rafael | Study Chair |
| Gabriela S Cruz, Msc | Hospital São Rafael | Study Chair |
| Ticiana F Larocca, MD, Msc | Hospital São Rafael | Study Chair |
| Márcia MN Rabelo, MD, Msc | Hospital São Rafael | Study Chair |
| Luís CL Correia, MD, PhD | Hospital São Rafael | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hospital São Rafael | Salvador | Estado de Bahia | 41253-190 | Brazil |
Not provided
| ID | Term |
|---|---|
| D014355 | Chagas Disease |
| ID | Term |
|---|---|
| D014352 | Trypanosomiasis |
| D056986 | Euglenozoa Infections |
| D011528 | Protozoan Infections |
| D010272 | Parasitic Diseases |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Plasma, Serum, Whole Blood
| D007239 |
| Infections |
| D000079426 | Vector Borne Diseases |