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| ID | Type | Description | Link |
|---|---|---|---|
| UL1TR001082 | U.S. NIH Grant/Contract | View source |
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The primary hypothesis of this proposal is that chronic kidney disease (CKD) and treatment with calcineurin inhibitors (CNIs) are each associated with the release of endothelial microparticles into the plasma.
The primary hypothesis of this proposal is that chronic kidney disease (CKD) and treatment with calcineurin inhibitors (CNIs) are each associated with the release of endothelial microparticles into the plasma. These injury-associated microparticles have a different molecular/protein composition than those released from endothelial cells in healthy patients, and their abundance correlates with vascular injury. To test this hypothesis the investigators propose a 3 arm case-controlled study including normal controls, individuals with stage III and IV CKD not receiving CNI therapy, and individuals with stage III and IV CKD post kidney transplantation receiving a CNI. The goal of this study is to measure the amount of microparticles in patients with kidney disease who have had a kidney transplant. Another goal of this study is to see if the measurements of microparticles are related to poor function of the vessels (endothelial dysfunction).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy control | Healthy control that meets inclusion/exclusion criteria. Single blood draw and flow-mediated dilation (FMD). |
| |
| Stage III or IV CKD patients | Stage III or IV CKD patients that meets inclusion/exclusion criteria. Single blood draw and flow-mediated dilation (FMD). |
| |
| Post-transplant patients | Stage III or IV CKD Post-transplant patients that meets inclusion/exclusion criteria. Single blood draw and flow-mediated dilation (FMD). |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Flow-mediated dilation | Other | Flow-mediated dilation used to measure vascular function. The test is minimally invasive, with only a slight discomfort due to the inflation of the arm cuff. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in Vascular Function | This will be measured with flow-mediated dilation and evaluate percent change from baseline over minutes. | percent change from baseline over 2 minutes |
| Measure | Description | Time Frame |
|---|---|---|
| Characterization of Microparticle source from blood analysis | A single blood draw to distinguish micro particle source characterization based upon protein, lipid and cholesterol composition. | Single time point |
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Inclusion Criteria:
For Healthy controls:
For Stage III or IV CKD patients:
For Post-kidney transplant and stage III or IV CKD patients:
Exclusion Criteria:
For Healthy controls:
-Women pregnant or breastfeeding
For Stage III or IV CKD patients:
For Post-transplant stage III or IV CKD patients:
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90 volunteers from the Anschutz Medical Campus
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| Name | Affiliation | Role |
|---|---|---|
| Joshua M Thurman, MD | University of Colorado, Denver | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Colorado Anschutz Medical Campus; CTRC | Aurora | Colorado | 80045 | United States | ||
| University of Colorado School of Medicine |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30006493 | Derived | Jalal D, Renner B, Laskowski J, Stites E, Cooper J, Valente K, You Z, Perrenoud L, Le Quintrec M, Muhamed I, Christians U, Klawitter J, Lindorfer MA, Taylor RP, Holers VM, Thurman JM. Endothelial Microparticles and Systemic Complement Activation in Patients With Chronic Kidney Disease. J Am Heart Assoc. 2018 Jul 13;7(14):e007818. doi: 10.1161/JAHA.117.007818. |
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| ID | Term |
|---|---|
| D051436 | Renal Insufficiency, Chronic |
| ID | Term |
|---|---|
| D051437 | Renal Insufficiency |
| D007674 | Kidney Diseases |
| D014570 | Urologic Diseases |
| D052776 | Female Urogenital Diseases |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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plasma and urine biospecimens to be retained.
| single blood draw | Other | single blood draw of approximately 45 mL. |
|
| Aurora |
| Colorado |
| 80045 |
| United States |
| D005261 |
| Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D052801 | Male Urogenital Diseases |
| D002908 | Chronic Disease |
| D020969 | Disease Attributes |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |