Potential Biomarkers of the Severity of Endothelial Damage in End-stage Chronic Kidney Failure

Potential Biomarkers of the Severity of Endothelial Damage in End-stage Chronic Kidney Failure Evaluated at the Initiation of Intermittent or Peritoneal Dialysis

The principal objective of this study is to qualify markers of oxidative stress in inflammatory cells (monocytes) in patients with stage 3 kidney failure (diabetic or not), and patients with end-stage kidney failure (diabetic or not), who require dialysis. The evaluation of these markers will be done by the activation and localization of proteins implicated in vascular tone and oxidative stress in monocytes, correlated with the distribution of cholesterol sphingomyelin within planar rafts and caveolae. The aim is to describe their evolution under treatment, which could lead to interventional studies.

Inclusion Criteria:

• Patient who have been informed about the research
• Patients with national health insurance cover
• Patients aged between 18 and 75 years
• Patients with stage 3 kidney failure (30 ml/min < cl creat < 59 ml/min) diabetic or not
• Patients with stage 5 kidney failure (cl creat < 15 ml/mn) diabetic or not and requiring dialysis.

Exclusion Criteria:

• Patients under guardianship
• Pregnant or breast-feeding women
• Infection (including peritonitis in peritoneal dialysis, infection of the catheter insertion site),
• Neoplastic disease,
• Systemic diseases in flare,
• Patients positive for Human Immunodeficience Virus (HIV),
• Patients on immunosuppressants
• Patients taking antioxidants (selenium, vitamin C and/or E)
• Patient on statins