Optimizing IVF Treatment - the Impact of Time-lapse Culture and Preimplantation Factor (PIF) on Embryo Development.

Optimizing IVF Treatment - the Impact of Time-lapse Culture and Preimplantation Factor (PIF) on Embryo Development.

The aims of this study are to evaluate:

1. the possible effect of time-lapse culture from day zero (day of oocyte retrieval) on fertilisation rates and embryo quality in standard IVF.
2. the association between PIF status in embryo culture medium and embryo quality assessed by standard subjective parameters and morphokinetic development.

Inclusion Criteria:

• Standard IVF treatment
• Body mass Index (BMI) between 18 and 30
• couples in which the male partner has normal sperm concentration and motility according to the World Health Organization (WHO, 2010)

Exclusion Criteria:

- Patients with less than four oocytes