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| ID | Type | Description | Link |
|---|---|---|---|
| TL1TR000066 | U.S. NIH Grant/Contract | View source | |
| UL1TR000064 | U.S. NIH Grant/Contract | View source | |
| 1P30AG028740 | U.S. NIH Grant/Contract | View source |
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| Name | Class |
|---|---|
| National Institutes of Health (NIH) | NIH |
| National Institute on Aging (NIA) | NIH |
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Healthy volunteers will be recruited to participate in a ten-week double-blinded crossover trial. The trial will consist of two, three-week periods of intermittent fasting, where subjects receive either antioxidant supplementation or placebo, the ordering of which will be randomly determined. A one-week preconditioning will precede each invention period, and a two week "wash-out" period will follow the first intervention period. Serum-based assays will be performed to assess levels of reactive oxidant species, antioxidant genes, sirtuins, and markers of mitochondrial biogenesis and aging.
The investigators hypothesize that an intermittent fasting diet in healthy young volunteers will improve these markers of cellular aging and that these beneficial effects will be abrogated by the supplementation of antioxidants. This study is a proof-of-principle study that will shed light on the mechanism and effects of IF as an anti-aging dietary intervention in the absence of weight loss. It will inform the design of dietary interventions that are both effective in improving markers of aging and feasible for patients to practice on a long-term basis.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Intermittent Fasting | Experimental | Intermittent Fasting |
|
| Intermittent Fasting + Antioxidants | Experimental | Intermittent Fasting; 400 IU Vitamin E; 1000 mg Vitamin C |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Intermittent Fasting | Other | The intermittent fasting paradigm used in this study will require participants to alternate between days of feasting (175% of normal caloric intake) and fasting ( 25% of normal caloric intake). Food will be provided by University of Florida Clinical Research Center with macronutrient composition prepared according to the 2010 Dietary Guidelines for Americans. |
| Measure | Description | Time Frame |
|---|---|---|
| SOD2 gene expression (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note: SOD2 = Superoxide-dismutase-2 | 3 weeks |
| SOD2 gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note: SOD2 = Superoxide-dismutase-2 | 3 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| GPx1 gene expression (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note: GPx1 = Glutathione peroxidase 1 | 3 weeks |
| SIRT1 gene expression (intermittent fasting) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Mark L Brantly, MD | University of Florida | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Florida | Gainesville | Florida | 32610 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 25546413 | Derived | Wegman MP, Guo MH, Bennion DM, Shankar MN, Chrzanowski SM, Goldberg LA, Xu J, Williams TA, Lu X, Hsu SI, Anton SD, Leeuwenburgh C, Brantly ML. Practicality of intermittent fasting in humans and its effect on oxidative stress and genes related to aging and metabolism. Rejuvenation Res. 2015 Apr;18(2):162-72. doi: 10.1089/rej.2014.1624. |
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| ID | Term |
|---|---|
| D000093763 | Intermittent Fasting |
| ID | Term |
|---|---|
| D005215 | Fasting |
| D005247 | Feeding Behavior |
| D001519 | Behavior |
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| ID | Term |
|---|---|
| D014810 | Vitamin E |
| D001205 | Ascorbic Acid |
| ID | Term |
|---|---|
| D001578 | Benzopyrans |
| D011714 | Pyrans |
| D006573 | Heterocyclic Compounds, 1-Ring |
| D006571 | Heterocyclic Compounds |
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|
| 400 IU Vitamin E | Dietary Supplement | once each day in morning; oral pill form |
|
| 1000 mg Vitamin C | Dietary Supplement | 500mg twice each day; morning and evening; oral pill form |
|
Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note SIRT1 = Sirtuin 1 |
| 3 weeks |
| SIRT3 gene expression (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note SIRT3 = Sirtuin 3 | 3 weeks |
| mTFA gene expression (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note: mTFA = mitochondrial transcription factor a | 3 weeks |
| NRF1 gene expression (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note NRF1 = Nuclear respiratory factor 1 | 3 weeks |
| 8oxodG ratio (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using DNA extracted from venous blood. Note: 8oxodG = 8-oxo-7,8-dihydro-2'-deoxyguanosine/2-deoxyguanosine | 3 weeks |
| 8oxoG ratio (intermittent fasting) | Observational outcome examines difference in expression between a normal diet and a 3-week intermittent fasting diet using RNA extracted from venous blood. Note: 8oxoG ratio = 8-oxo-7,8 dihydroguanosine/guanosine | 3 weeks |
| GPx1 gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note: GPx1 = Glutathione peroxidase 1 | 3 weeks |
| SIRT1 gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note SIRT1 = Sirtuin 1 | 3 weeks |
| SIRT3 gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note SIRT3 = Sirtuin 3 | 3 weeks |
| mFTA gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note: mTFA = mitochondrial transcription factor a | 3 weeks |
| NRF1 gene expression (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note NRF1 = Nuclear respiratory factor 1 | 3 weeks |
| 8oxodG ratio (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using DNA extracted from venous blood. Note: 8oxodG ratio = 8-oxo-7,8-dihydro-2'-deoxyguanosine/2-deoxyguanosine | 3 weeks |
| 8oxoG ratio (antioxidant supplementation) | Experimental outcome examining differences between intermittent fasting with supplementation of antioxidants and intermittent fasting alone using RNA extracted from venous blood. Note: 8oxoG ratio = 8-oxo-7,8 dihydroguanosine/guanosine | 3 weeks |
| D006574 |
| Heterocyclic Compounds, 2-Ring |
| D000072471 | Heterocyclic Compounds, Fused-Ring |
| D013400 | Sugar Acids |
| D000144 | Acids, Acyclic |
| D002264 | Carboxylic Acids |
| D009930 | Organic Chemicals |
| D006880 | Hydroxy Acids |
| D002241 | Carbohydrates |