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The aim of this study is to investigate the impact of renal function and dialysis techniques on the percentage of senescent T lymphocytes.
The immunosenescence is a complex and profound remodeling of the immune system during life. It is mainly due to thymic involution and repeated antigenic stimulation. Kidney disease is associated with a decrease in adaptive immunity as evidenced by the decrease in vaccine response and increased susceptibility to infections, similar to those observed in the elderly population.
However, data on aging of the immune system in chronic kidney disease remains incomplete. Furthermore, the determinants of immunosenescence are not also not known. It is possible that "uremic" factors help explain the phenotypic and functional changes of lymphocytes, as antigenic stimuli associated with repeated bio-compatible materials used in dialysis contact.
The purpose of this study is to describe the phenotypes of the immune system of renal and analyze the determinants of these changes.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control group | Other | Patients in this group control (normal renal function) are followed in the urology department. A blood sample is performed on the day of inclusion. |
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| Severe renal failure | Experimental | Patients in this group suffer from renal failure stage 4 and are not under dialysis. A blood sample is performed on the day of inclusion. |
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| Peritoneal dialysis | Experimental | Patients with renal failure, under peritoneal dialysis for at least 3 months. A blood sample is performed on the day of inclusion. |
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| Hemodialysis | Experimental | Patient with renal failure, under hemodialysis for at least 3 months. A blood sample is performed on the day of inclusion. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood sample | Biological | 3 tubes of 5 ml tubes and 4 of 7 ml for biological assays at t0. |
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| Measure | Description | Time Frame |
|---|---|---|
| Percentage of Cluster of Differentiation (CD) 4/8+ CD 57+ CD 28- lymphocytes (senescent lymphocytes) | The primary outcome measure is the percentage of CD 4/8+ CD 57+ CD 28- lymphocytes by flow cytometry. The technique used is a 6 colors surface labelling of T lymphocytes to study the T cell senescent population. | 6 months |
| Measure | Description | Time Frame |
|---|---|---|
| Telomerase Activity of T lymphocytes | The telomerase activity of T lymphocytes is assessed by a method of Polymerase chain reaction (PCR)-ELISA. | 6 months |
| Level of phospho-histone 2AX (gH2AX) in peripheral blood T lymphocytes |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Jamal BAMOULID, Doctor | CHU de Besançon | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Service de néphrologie, CHU de Besançon | Besançon | 25000 | France |
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| ID | Term |
|---|---|
| D051437 | Renal Insufficiency |
| ID | Term |
|---|---|
| D007674 | Kidney Diseases |
| D014570 | Urologic Diseases |
| D052776 | Female Urogenital Diseases |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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This level is obtained by flow cytometry after permeabilization and labelling of Peripheral Blood Mononuclear Cells (PBMC).
| 6 months |
| Proportion of Recent Thymic Emigrants (RTE) in peripheral blood T lymphocytes | RTE T cells will be defined by flow cytometry, according to co-expression of CD 4, CD 8,CD 31 and CD 45RA | 6 months |
| T-cell receptor excision circle (TREC) level in PBMC. | TRECs study is based on a technique of quantitative PCR using DNA extracted from PBMC. | 6 months |
| Telomere length in T lymphocytes | Study of telomere length is performed after extraction of DNA from isolated T cells. The length is determined by a quantitative PCR technique relative to a reference gene. | 6 months |
| D000091642 | Urogenital Diseases |
| D052801 | Male Urogenital Diseases |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |