Induction of Pluripotent Stem Cells From Human Fibroblasts of DM1 Patients

Induction of Pluripotent Stem Cells From Human Fibroblasts of DM1 Patients

The objective of the study is the modelisation of human cardiomyocyts from pluripotent stem cells in order to study the cardiac alterations induced by CTG expansions.

Inclusion Criteria for DM1 patient with cardiomyopathy:

• aged 20-50
• DM1 confirmed by molecular analysis
• Confirmed cardiomyopathy (infrahissiens conduction disorders)
• able to sign the consent form
• affiliated to european social security.

Inclusion Criteria for DM1 patient without cardiomyopathy

• aged 20-50
• DM1 confirmed by molecular analysis
• No cardiomyopathy (no infrahissiens conduction disorders or other trouble linked to DM1)
• able to sign the consent form
• affiliated to european social security.

Inclusion Criteria for controls

• aged over 18
• brother, sister, father or mother of the DM1 patient
• No DM1 confirmed by molecular analysis (CTG repetition under 38)
• No cardiac disorders.
• able to sign the consent form
• affiliated to european social security.

Exclusion Criteria:

• no pragnant or nursing women
• patient unable to understand informed consent
• patient under juridic protection