To assess normal values of EGJ distensibility in healthy volunteers.

Inclusion Criteria:

1. No history of gastrointestinal disease, especially gastro-esophageal reflux disease
2. BMI = 18-30 kg/m²
3. Caucasian race
4. Subject signed the informed consent form and is able to adhere to study protocol

Exclusion Criteria:

1. Age < 18 years
2. Previous esophageal or gastric surgery
3. Co-morbidities (including cardiopulmonary disease, collagen diseases, obesity, coagulation disorders and co-morbidity hindering upper gastrointestinal endoscopy)
4. Patients using anticoagulants
5. Patients unable to give informed consent
6. Smoking
7. History of alcohol abuse or current excessive alcohol consumption (> 2 alcoholic beverages per day or > 14 alcoholic beverages per week)

15 healthy volunteers, age > years