Immunophenotyping of Fresh Stromal Vascular Fraction From Adipose Derived Stem Cells (ADSC) Enriched Fat Grafts

Immunophenotyping of Fresh Stromal Vascular Fraction From Adipose Derived Stem Cells (ADSC) Enriched Fat Grafts for Refinements of Reconstructed Breasts

The purpose of this study is to investigate if there is a relationship between the take of fat grafts with and without ADSCs and the presence of specific surface markers on the cells of the stromal vascular fraction.

Two groups of patients will be studied. The control will receive centrifuged fat grafts while the other group will receive centrifuged enriched fat grafts with ADSCs. The performance of both grafts will be assessed through volume measurement employing MRI before and after 6 months post-op.

Inclusion Criteria:

• contour irregularities and volume insufficiency in reconstructed breasts no matter the method of reconstruction employed
• local flaps with conditions to receive fat grafts
• good health condition

Exclusion Criteria:

• breast cancer patients under chemotherapy
• smokers
• bad health condition
• patients too thin
• patients that require a new reconstructive surgery (secondary reconstruction)