The purpose of this study is to determine whether laser-assisted zona thinning improve the implantation rate of the women of advanced age in the first IVF/ICSI cycles

Registry procedures：

1. It's required to sign an informed consent form prior to the recruitment of all patients.
2. Data collection and entry need double check and supervision.

The outcome measures:

the implantation rate per embryo transfer miscarriage live birth

Inclusion Criteria:

• Age: 35y ≤age≤46y
• Basal FSH value: FSH≤10IU/L
• Day-3 embryos transferred in the fresh IVF/ICSI cycles
• the number of embryos available to be transferred on Day-3: ≥one embryo

Exclusion Criteria:

• cycles with thawed/warmed embryos biopsied for PGD/PGS.
• cycles with oocyte donation transfer.
• the maximum number of previous unsuccessful IVF attempts: ≥ 3 cycles(transfer cycles)
• any cycles with the risk of OHSS, fluid in the uterine cavity, abnormal endometrium, uterine abnormalities,pelvic inflammation, and other acute maternal problems.
• One or both spouses have an abnormal karyotype (including polymorphism)