Aim of this study is to provide specimens for establishing reference values of HE4 in an apparently healthy Chinese population using the Roche Elecsys HE4 assay tested on variety of Elecsys and cobas brand immunoassay analyzers. This reference range will be compared to population of subjects who have signs and symptoms of various benign and malignant diseases, including ovarian cancer. This reference range will also be compared to the levels of HE4 in individual subjects who have been diagnosed with ovarian cancer and are being followed longitudinally for recurrence or progression of their disease.

Inclusion Criteria (all cohorts):

• appropriate clinical data
• normal appearance of sample
• minimum 0.5 ml volume available

Exclusion Criteria (all cohorts):

• < 18 years of age
• insufficient volume
• insufficient clinical information
• > 3 freeze/thaw cycles of samples
• Stored or shipped at > 0°C
• Icteric, lipemic, hemolytic, substantial particulates

Except for samples from apparently healthy subjects, specimens for the entire clinical section are supposed to be available in banks / archives. Thus, for the most part, no prospective collection of serum should be necessary. Pseudonymized residual samples will be used. All elements of the clinical part require samples from well characterized cohorts are showed below.

1. Apparently Healthy: 300
2. Non-malignant Conditions: 650
3. Malignant Conditions: 800