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Direct DNA amplification, viral isolation and specific antibody measurement in saliva from children attending nursery in France Sample collection will be done by the pediatrician the nurse, or one of the authorized investigators of the study.
Salivary collection kit. 3 swab for DNA research, 1 for antibodies research and 1 for virus isolation by cell growth.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Child in nursery | Children older than 3 months and less than 6 years kept in nurseries retained, regardless of the duration of custody of the child in the manger concerned. |
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| Measure | Description | Time Frame |
|---|---|---|
| Identification of viral shedding | Saliva using small sponges, non-invasive for the viral genome, isolating virus strains and measurement of antibody IgG and IgA. | 1 day |
| Measure | Description | Time Frame |
|---|---|---|
| Evaluate the distribution of different genotypes of strains of CMV | distribution by genotype strains found | 1 day |
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Inclusion Criteria:
Exclusion Criteria:
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Children older than 3 months and less than 6 years kept in nurseries retained, regardless of the duration of custody of the child in the manger concerned.
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| Name | Affiliation | Role |
|---|---|---|
| Sophie ALAIN, MD | Limoges UH | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Bacteriology | Limoges | 87042 | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 32068854 | Derived | Alain S, Garnier-Geoffroy F, Labrunie A, Montane A, Marin B, Gatet M, Grosjean J, Dufour V, Saugeras M, Postil D, Hantz S. Cytomegalovirus (CMV) Shedding in French Day-Care Centers: A Nationwide Study of Epidemiology, Risk Factors, Centers' Practices, and Parents' Awareness of CMV. J Pediatric Infect Dis Soc. 2020 Dec 31;9(6):686-694. doi: 10.1093/jpids/piz097. |
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| ID | Term |
|---|---|
| D007239 | Infections |
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Direct DNA amplification, viral isolation and specific antibody measurement in saliva