Detection of Human Cytomegalovirus in the Saliva

Detection of Human Cytomegalovirus in the Saliva of 3 Month to 6 Year-old Children in Day Care Centers : a Pilot Study.

Direct DNA amplification, viral isolation and specific antibody measurement in saliva

1. From children in day care centers (samples collection will take place during a single week)
2. From children coming at the emergency department of our hospital during a three months period.

Sample collection will be done by the pediatrician the nurse, or one of the authorized investigators of the study.

Salivary collection kit. Collection of salivary at the internal face of the mooth. 3 swab for DNA research, 1 for antibodies research and 1 for virus isolation by cell growth.

Inclusion Criteria:

• parents consent
• child aged from 3months to 6 years
• child in day care centers or emergency department

Exclusion Criteria:

• Refuse of participation