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| ID | Type | Description | Link |
|---|---|---|---|
| 91611074 | Other Grant/Funding Number | The Netherlands Organisation for Scientific Research |
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| Name | Class |
|---|---|
| Netherlands Organisation for Scientific Research | OTHER_GOV |
| European Foundation for the Study of Diabetes | OTHER |
Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| NGT | Normal glycaemic healthy control men, age between 45-65, BMI 25-35 kg/m2 |
| |
| T2D | Type 2 diabetic men, age 45-65 yrs, BMI 25-35, diagnosed >5yrs and Hba1c 7-9% |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Hyperinsulinemic euglycemic clamp | Other |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis | Changes from baseline in glycerol (lactate, pyruvate and glucose) in tissue microdialysate during a 3 step euglycemic hyperinsulinemic clamp (8, 20 and 40mU of insulin), every step for 2h | 8, 20 and 40 mU insulin (2 hours) |
| Measure | Description | Time Frame |
|---|---|---|
| Baseline adipose tissue and skeletal muscle gene expression | gene expression of genes related to lipid, glucose metabolism, insulin signalling will be measured in baseline adipose tissue and skeletal muscle biopsies | baseline |
| Baseline skeletal muscle lipid accumulation |
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Inclusion Criteria:
Exclusion Criteria:
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Healthy normoglycemic men and type 2 diabetic men
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| Name | Affiliation | Role |
|---|---|---|
| Ellen Blaak, PhD | Maastricht University Medical centre | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Human Biology, Maastricht University Medical Centre | Maastricht | 6200MD | Netherlands |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 20843949 | Background | Jocken JW, Moro C, Goossens GH, Hansen D, Mairal A, Hesselink MK, Langin D, van Loon LJ, Blaak EE. Skeletal muscle lipase content and activity in obesity and type 2 diabetes. J Clin Endocrinol Metab. 2010 Dec;95(12):5449-53. doi: 10.1210/jc.2010-0776. Epub 2010 Sep 15. | |
| 18398140 | Background | Jocken JW, Roepstorff C, Goossens GH, van der Baan P, van Baak M, Saris WH, Kiens B, Blaak EE. Hormone-sensitive lipase serine phosphorylation and glycerol exchange across skeletal muscle in lean and obese subjects: effect of beta-adrenergic stimulation. Diabetes. 2008 Jul;57(7):1834-41. doi: 10.2337/db07-0857. Epub 2008 Apr 8. |
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| ID | Term |
|---|---|
| D003924 | Diabetes Mellitus, Type 2 |
| ID | Term |
|---|---|
| D003920 | Diabetes Mellitus |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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adipose tissue and skeletal muscle biopsies
Baseline skeletal muscle lipid accumulation is measured (TAG, DAG, Phospholipid and fatty acids) |
| Baseline |
| 18060661 | Background | Jocken JW, Goossens GH, van Hees AM, Frayn KN, van Baak M, Stegen J, Pakbiers MT, Saris WH, Blaak EE. Effect of beta-adrenergic stimulation on whole-body and abdominal subcutaneous adipose tissue lipolysis in lean and obese men. Diabetologia. 2008 Feb;51(2):320-7. doi: 10.1007/s00125-007-0866-y. Epub 2007 Dec 5. |
| 17131144 | Background | Boon H, Blaak EE, Saris WH, Keizer HA, Wagenmakers AJ, van Loon LJ. Substrate source utilisation in long-term diagnosed type 2 diabetes patients at rest, and during exercise and subsequent recovery. Diabetologia. 2007 Jan;50(1):103-12. doi: 10.1007/s00125-006-0482-2. Epub 2006 Nov 28. |
| 23907381 | Derived | Jocken JW, Goossens GH, Boon H, Mason RR, Essers Y, Havekes B, Watt MJ, van Loon LJ, Blaak EE. Insulin-mediated suppression of lipolysis in adipose tissue and skeletal muscle of obese type 2 diabetic men and men with normal glucose tolerance. Diabetologia. 2013 Oct;56(10):2255-65. doi: 10.1007/s00125-013-2995-9. Epub 2013 Aug 2. |
| D004700 | Endocrine System Diseases |