Regulation of Tissue Lipolysis by Insulin in Type 2 Diabetes

Regulation of Lipolysis by Insulin in Skeletal Muscle and Adipose Tissue in Type 2 Diabetes

Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.

Inclusion Criteria:

• Caucasian
• Normal blood pressure (SBP 100-140 mmHg, DBP 60-90 mmHg)
• weight stable in last 3 months

Exclusion Criteria:

• Smokers
• people with intensive fitness training (e.g. athletes > 3 times/week)
• History of cardiovascular diseases
• Bleeding disorders
• Use of medication interfering with the study endpoints/hypotheses (e.g. beta-blockers)
• Not to be able to understand the study information
• Subjects on a special diet or vegetarian
• Blood donation 2 months prior to the study and during the study
• Participating in an other study