This trial is conducted in Europe. The aim of this trial is to demonstrate a difference between the pharmacodynamics and pharmacokinetics of biphasic insulin aspart 30, biphasic insulin aspart 50, biphasic insulin aspart 70 and insulin aspart in healthy subjects.

Inclusion Criteria:

• Body mass index between 18 and 28 kg/m^2 inclusive
• HbA1c within the normal laboratory range
• Non smoker for at least three months
• Females of childbearing potential using acceptable methods of contraception, including tubal ligation, an intrauterine device (IUD) , the oral contraceptive pill or barrier methods

Exclusion Criteria:

• Subjects who have received any investigational drug in the 3 months prior to the start of dosing
• Any disease requiring regular use of non topical prescription medicines
• Any serious systemic infectious disease that occurred in the 4 weeks prior to the first dose of test drug
• Any intercurrent illness or endocrine disorders that may affect blood glucose
• Subject with a history of drug or alcohol dependence
• Subject with a first degree relative with diabetes mellitus
• Subject with a history of clinically relevant allergic reactions to medical products
• Subjects who have donated any blood or plasma in the past 3 month preceding screening