Long-term Investigation of Resveratrol on Fat Metabolism in Obese Men With Nonalcoholic Fatty Liver Disease

Long-term Investigation of Resveratrol on Lipid Turnover in Obese Men With Nonalcoholic Fatty Liver Disease. Effects on Liver Fat Content and Basal and Insulin Stimulated FFA and VLDL-triglyceride Metabolism

The purpose of this study is to investigate potential metabolic effects of resveratrol in obese healthy men with non-alcoholic fatty liver disease.

The investigators hypothesize that resveratrol will:

• decrease hepatic very-low-density-lipoprotein-triglyceride (VLDL-TG) secretion
• decrease liver fat content
• increase insulin sensitivity

The investigators will look at changes in:

• lipid turnover (VLDL-TG kinetics, palmitate kinetics, indirect calorimetry)
• liver fat content (MR liver spectroscopy)
• insulin sensitivity (glucose kinetics during hyperinsulinaemic euglycaemic clamp)
• body composition (DXA and MRI)
• lipase activity and fat cell size (fat biopsy from abdominal and femoral adipose tissue)

Inclusion Criteria:

• Male
• 25-65 years
• Obesity (BMI > 28 kg/m2, waist/hip ratio > 0,95)
• Have nonalcoholic fatty liver disease (NAFLD)(intervention group) or do not have NAFLD (control group)
• May have hypertension and/or hypercholesterolemia
• Written informed consent

Exclusion Criteria:

• Any other disease than NAFLD (e.g. diabetes, thyroid or parathyroid disease, heart, liver or kidney disease)
• Present and previous malignancy
• Alcohol dependency (more than 21 units of alcohol per week)
• History of smoking
• Participation in studies with radioactive isotopes within the last six months
• Hemoglobin under normal range regarding to sex (under 8.3 mmol/l for men)