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| ID | Type | Description | Link |
|---|---|---|---|
| COG-ANBL11B1 | Other Identifier | Children's Oncology Group | |
| NCI-2011-02862 | Registry Identifier | CTRP (Clinical Trial Reporting Program) | |
| ANBL11B1 | Other Identifier | Children's Oncology Group |
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| Name | Class |
|---|---|
| National Cancer Institute (NCI) | NIH |
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RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research trial studies biomarkers in blood and tissue samples from patients with newly diagnosed neuroblastoma.
OBJECTIVES:
OUTLINE: Archived tumor tissue and serum samples are analyzed for neuropeptide Y and its receptors (Y1, Y2, and Y5) expression, neuroblastoma prognostic factors (MYCN, TrkA, TrkAIII, TrkB, BDNF, and NGF), and angiogenic markers by real-time PCR, IHC, ELISA, radioimmunoassay (RIA), mitogenic assay, caspase 3/7 activity assay, western blots, liquid chromatography, tandem mass spectrometry, proteomic assays, and other assays. Results are then analyzed and compared with patients' clinical data, including stage of disease, its phenotype, prognostic markers, age and gender, and response to treatment.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| RNA analysis | Genetic | |||
| polymerase chain reaction | Genetic | |||
| protein expression analysis | Genetic | |||
| proteomic profiling | Genetic | |||
| western blotting | Genetic | |||
| enzyme-linked immunosorbent assay | Other | |||
| immunohistochemistry staining method | Other |
| Measure | Description | Time Frame |
|---|---|---|
| Association of high expression of NPY and its Y2/Y5 Rs in NBs with poor outcome of the disease, advanced stage, increased vascularization and other unfavorable prognostic factors, such as TrkB expression and MYCN amplification | ||
| BDNF/TrkB and TrkAIII up-regulate expression of NPY and its Rs | ||
| NPY upregulates expression of the identified proteins in NB and their Rs in endothelial cells (ECs) | ||
| Interaction between Y2 and Y5 receptors in NB and ECs sensitize them to NPY and amplify NPY-induced proliferation | ||
| Blocking the NPY-Y2/Y5 pathway reduces NB growth and tumor vascularization |
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DISEASE CHARACTERISTICS:
Children with newly diagnosed neuroblastoma or ganglioneuroblastoma
Samples from the Children's Oncology Group (COG) from patients enrolled in clinical trials in institutions in the USA, Canada, and Australia
PATIENT CHARACTERISTICS:
PRIOR CONCURRENT THERAPY:
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Children with newly diagnosed neuroblastoma or ganglioneuroblastoma.
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| Name | Affiliation | Role |
|---|---|---|
| Joanna Kitlinska, PhD | Lombardi Comprehensive Cancer Center | Principal Investigator |
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blood and tissue
| laboratory biomarker analysis | Other |
| liquid chromatography | Other |
| mass spectrometry | Other |
| ID | Term |
|---|---|
| D009447 | Neuroblastoma |
| ID | Term |
|---|---|
| D018241 | Neuroectodermal Tumors, Primitive, Peripheral |
| D018242 | Neuroectodermal Tumors, Primitive |
| D018302 | Neoplasms, Neuroepithelial |
| D017599 | Neuroectodermal Tumors |
| D009373 | Neoplasms, Germ Cell and Embryonal |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
| D009375 | Neoplasms, Glandular and Epithelial |
| D009380 | Neoplasms, Nerve Tissue |
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| ID | Term |
|---|---|
| D016133 | Polymerase Chain Reaction |
| D015153 | Blotting, Western |
| D004797 | Enzyme-Linked Immunosorbent Assay |
| D007150 | Immunohistochemistry |
| D002853 | Chromatography, Liquid |
| D013058 | Mass Spectrometry |
| ID | Term |
|---|---|
| D021141 | Nucleic Acid Amplification Techniques |
| D005821 | Genetic Techniques |
| D008919 | Investigative Techniques |
| D004586 | Electrophoresis |
| D002623 | Chemistry Techniques, Analytical |
| D055664 | Electrochemical Techniques |
| D015151 | Immunoblotting |
| D007118 | Immunoassay |
| D007158 | Immunologic Techniques |
| D015336 | Molecular Probe Techniques |
| D007124 | Immunoenzyme Techniques |
| D007163 | Immunosorbent Techniques |
| D006651 | Histocytochemistry |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D006652 | Histological Techniques |
| D002845 | Chromatography |
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