PURPOSE

The goal of the present project is to unravel the importance of bile acid-induced GLP-1 secretion (via the G protein-coupled receptor TGR5) in human physiology.

In the present study the investigators examine the GLP-1 secretion in healthy subjects and cholecystectomised patients. Thereby the investigators aim to show, that cholecystectomised patients have reduced bileflow after a meal, and as a consequence exhibit a diminished GLP-1 response as compared to healthy subjects.

Inclusion Criteria: (Healthy)

• Normal fasting plasma glucose
• Normal glucose tolerance
• Normal haemoglobin levels
• Informed content

Inclusion Criteria: (cholecystectomy group)

• Laparoscopic cholecystectomy <6 months. Indication: Gallstones.
• Normal fasting plasma glucose
• Normal glucose tolerance
• Normal haemoglobin levels
• Informed content

Exclusion Criteria: (both groups)

• Diabetes
• Pre diabetes (impaired glucose tolerance or impaired fasting glucose)
• 1st degree relatives with diabetes
• Obesity (BMI > 30)
• Inflammatory bowels disease
• Nephropathy
• Liver disease
• Medication which cannot be on hold for 24h

Healthy subjects and cholcystectomised patients (chol)