PD-1/PD-L1 Pathway Study on Septic Patients

PD-1/PD-L1 Pathway in T Lymphocyte Apoptosis and Monocyte Dysfunction in Septic Patients

The investigators want to identify that whether PD-1/PD-L1 pathway will change in human sepsis, and whether PD-1/PD-L1 pathway play an important role in sepsis induced immunosuppression.

First, we will detect PD-L1 expression on PBMC of septic patients and healthy controls.

Second, blockade of PD-1/PD-L1 pathway in vitro and stimulate with some biomolecules and then analyze the PBMC function and apoptosis by flow cytometry.

Then, compare with healthy controls and find the differences.

Inclusion Criteria:

• patients who develop sepsis

Exclusion Criteria:

• patients with cirrhosis or renal dysfunction
• patients with dyscrasia
• patients with autoimmunity diseases
• patients with severe cardiac and pulmonary diseases
• patients with hematological diseases
• patients with diabetes mellitus
• patients with malignant tumors