Role of microRNA in the Development of Cutaneous Squamous Cell Carcinoma

The aim of this study is to analyze the differential expression of the miR transcriptome in the distinctive stages of the development of cutaneous squamous cell carcinoma (C-SCC). In this aim the investigators plan to recruit a cohort of 20 patients suffering of C-SCC and to collect from each of them, biopsies corresponding to i) non UV-exposed areas ii) UV-exposed areas, iii) actinic keratosis and iv) tumoral regions. Total RNAs will be prepared from each biopsy and the miRNA profiles will be characterized using a dedicated miR array.

Inclusion Criteria:

• patient older than 40th, suffering of a C-SCC
• letter of consent signed by the patient
• to be registered to social security

Exclusion Criteria:

• Pregnant women or breastfeeding.
• Small C-SCC (size < 6mm)
• Allergy to Xylocaine
• All vulnerable (minor, adult guardianship…)