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| ID | Type | Description | Link |
|---|---|---|---|
| COG-AOST10B4 | Other Identifier | Children's Oncology Group | |
| CDR0000674830 | Other Identifier | Clinical Trials.gov | |
| NCI-2011-02234 | Registry Identifier | CTRP (Clinical Trial Reporting Program) |
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| Name | Class |
|---|---|
| National Cancer Institute (NCI) | NIH |
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RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is studying DNA biomarkers in samples from patients with osteosarcoma and healthy volunteers.
OBJECTIVES:
OUTLINE: RNA and DNA samples from banked blood and paired tumor tissue, plus samples from healthy controls, are analyzed for common copy-number alterations and constitutional copy-number variations (CNVs) at chr7p14.1 by microarray, q-PCR, RT-PCR, and FISH. Osteosarcoma predisposing CNVs results are then compared among cases versus healthy controls.
Clinical information associated with each osteosarcoma sample (i.e., gender, age of diagnosis, tumor site, tumor type and grade, presence of metastases at time of diagnosis, response to chemotherapy, event-free survival, and overall survival) is also collected, if available.
PROJECTED ACCRUAL: A total of 243 samples from patients with osteosarcoma and 80 samples from healthy controls will be accrued to this study.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| DNA analysis | Genetic | |||
| RNA analysis | Genetic | |||
| fluorescence in situ hybridization | Genetic | |||
| microarray analysis | Genetic | |||
| polymerase chain reaction | Genetic | |||
| reverse transcriptase-polymerase chain reaction | Genetic | |||
| laboratory biomarker analysis | Other |
| Measure | Description | Time Frame |
|---|---|---|
| Role of copy-number alterations (CNAs) in the etiology of osteosarcoma | ||
| Association between copy-number variations (CNVs) at chr7p14.1 and susceptibility to osteosarcoma | ||
| Relationship between CNVs and tumor CNAs in osteosarcoma |
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DISEASE CHARACTERISTICS:
Meets 1 of the following criteria:
Diagnosis of osteosarcoma (OS) and meets 1 of the following criteria:
Original 153 OS samples, including paired germline and tumor DNA
Additional samples from 90 patients with OS:
Healthy controls, age- and gender-matched
PATIENT CHARACTERISTICS:
PRIOR CONCURRENT THERAPY:
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Diagnosis of osteosarcoma
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| Name | Affiliation | Role |
|---|---|---|
| David Malkin, MD | The Hospital for Sick Children | Principal Investigator |
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| ID | Term |
|---|---|
| D012509 | Sarcoma |
| D012516 | Osteosarcoma |
| ID | Term |
|---|---|
| D018204 | Neoplasms, Connective and Soft Tissue |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
| D018213 | Neoplasms, Bone Tissue |
| D009372 | Neoplasms, Connective Tissue |
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| ID | Term |
|---|---|
| D017404 | In Situ Hybridization, Fluorescence |
| D046228 | Microarray Analysis |
| D016133 | Polymerase Chain Reaction |
| D020133 | Reverse Transcriptase Polymerase Chain Reaction |
| ID | Term |
|---|---|
| D017403 | In Situ Hybridization |
| D013194 | Staining and Labeling |
| D016591 | Histocytological Preparation Techniques |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D006652 | Histological Techniques |
| D008919 | Investigative Techniques |
| D020732 | Cytogenetic Analysis |
| D005821 | Genetic Techniques |
| D009693 | Nucleic Acid Hybridization |
| D046208 | Microchip Analytical Procedures |
| D021141 | Nucleic Acid Amplification Techniques |
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